Pinto H A, Melo A L
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
J Parasitol. 2013 Aug;99(4):729-33. doi: 10.1645/12-13.1. Epub 2013 Jan 29.
Austrodiplostomum compactum has been involved in cases of ocular diplostomiasis in several species of fish in Brazil, but the molluscan intermediate hosts of the parasite remain unknown. In the present study, malacological surveys were carried out at Pampulha Reservoir, Belo Horizonte, Minas Gerais, Brazil, between January 2009 and July 2012. A total of 16,119 specimens of Biomphalaria spp. were collected and examined, of which 68/14,948 specimens (0.45%) of Biomphalaria straminea and 6/541 (1.11%) of Biomphalaria glabrata were found harboring a strigeid cercariae. Groups of 5 specimens of Cyprinius carpio were experimentally infected with these cercariae (100 larvae/fish), and metacercariae were recovered from the eyes of the fish, 65 days after infection, with a mean intensity of infection of 10.4 (8-13) metacercariae/fish. Morphological study on cercariae and metacercariae identified them as A. compactum . This is the first record of cercariae of A. compactum in Brazil, and B. straminea and B. glabrata as new intermediate hosts for the parasite.
紧密澳双穴吸虫已在巴西多种鱼类的眼双穴吸虫病病例中出现，但该寄生虫的软体动物中间宿主仍不清楚。在本研究中，于2009年1月至2012年7月期间在巴西米纳斯吉拉斯州贝洛奥里藏特市的潘普利亚水库开展了软体动物学调查。总共收集并检查了16119只双脐螺属标本，其中发现14948只淡黄双脐螺标本中有68只（0.45%）、541只光滑双脐螺标本中有6只（1.11%）携带有一种三肠科尾蚴。将5组鲤鱼（每组5条）用这些尾蚴进行实验性感染（每条鱼100只幼虫），感染65天后从鱼眼中回收了后尾蚴，平均感染强度为每条鱼10.4（8 - 13）个后尾蚴。对尾蚴和后尾蚴的形态学研究将它们鉴定为紧密澳双穴吸虫。这是紧密澳双穴吸虫尾蚴在巴西的首次记录，以及淡黄双脐螺和光滑双脐螺作为该寄生虫新中间宿主的首次记录。
