毒液衍生肽 (ICD-85) 的体内和体外抗血管生成作用。

In vivo and in vitro anti-angiogenesis effect of venom-derived peptides (ICD-85).

机构信息

Department of Biochemistry, Payam Noor University of Mashhad, Mashhad, Iran.

出版信息

Arch Iran Med. 2013 Feb;16(2):109-13.

Abstract

BACKGROUND

Agiogenesis is the development of new blood vessels from pre-existing vasculatures. Although essential in the physiological process, it becomes pathological in various diseases including cancer. Preventing the formation of new blood vessels causes reductions in tumor size and metastasis. This study has been undertaken to elucidate the anti-angiogenesis effects of ICD-85 (derived peptides from venom).

METHODS

We evaluated the ICD-85 anti-angiogenesis activity by the in vivo CAM assay and in vitro tube formation assay of human umbilical vein endothelial cells (HUVECs). The anti-proliferative activity of ICD-85 was also determined through MTT assay on HUVECs.

RESULTS

Results of this study revealed the anti-proliferative activity of ICD-85 on the HUVEC cell line with an IC50 of 12 μg/mL. The in vivo CAM assay also clearly showed the prevention of new vascular formation when the chick embryos were exposed to 0.15 µg/disc of ICD-85. In vitro tube formation assay of HUVECs also showed the complete prevention of capillary tube formation on 18 µg/mL.

CONCLUSION

Based on the results obtained in this study, ICD-85 has anti-angiogenesis activity as shown by the prevention of capillary tube formation and the CAM assay.

摘要

背景

血管生成是指从预先存在的脉管系统中发展出新的血管。尽管它在生理过程中是必不可少的，但在包括癌症在内的各种疾病中会变得病态。阻止新血管的形成会导致肿瘤缩小和转移减少。本研究旨在阐明 ICD-85（源自毒液的衍生肽）的抗血管生成作用。

方法

我们通过体内 CAM 测定和人脐静脉内皮细胞（HUVEC）的体外管形成测定来评估 ICD-85 的抗血管生成活性。通过 MTT 测定法还确定了 ICD-85 对 HUVEC 的增殖活性。

结果

本研究的结果表明，ICD-85 对 HUVEC 细胞系具有增殖活性，IC50 为 12 μg/mL。体内 CAM 测定也清楚地表明，当鸡胚暴露于 0.15 µg/disc 的 ICD-85 时，新血管形成得到预防。HUVEC 的体外管形成测定也表明，在 18 µg/mL 时完全阻止了毛细血管管形成。