钡离子对单层培养并由胎鼠脊髓支配的人体肌肉的影响。

The effect of Ba ions on human muscle cultured in monolayer and innervated with fetal rat spinal cord.

作者信息

Kobayashi T, Michikawa M, Miyazaki H, Tsukagoshi H

机构信息

Department of Neurology, Tokyo Medical and Dental University, Japan.

出版信息

Neurosci Lett. 1990 Mar 26;111(1-2):157-63. doi: 10.1016/0304-3940(90)90361-c.

DOI:10.1016/0304-3940(90)90361-c

PMID:2336181

Abstract

In human muscle culture, 7% of aneurally (AMs) and 5.9% of innervated contracting muscle cells (ICMs) showed slow repolarization components (SRCs: duration, less than 10 ms) of action potentials. After an application of 10 mM Ba ion, prolonged SRCs, which were blocked by nifedipine, appeared in 96% of the AMs and 70% of the ICMs. The duration of SRCs under the application of 10 mM Ba solution were not significantly different between AM and ICM (2.8 and 2.0 s, respectively). The resting membrane potentials (RMPs) of AMs and ICMs decreased to 78% and 74% in the medium with 10 mM Ba solution, respectively, and the input resistance (Rin) of AM increased to 161% in the medium with 10 mM Ba. Slow hyperpolarizing afterpotentials were observed both in AMs and ICMs, and completely disappeared during the application of Ba.

摘要

在人类肌肉培养中，7%的去神经肌肉细胞（AMs）和5.9%的有神经支配的收缩性肌肉细胞（ICMs）表现出动作电位的缓慢复极化成分（SRCs：持续时间小于10毫秒）。施加10 mM Ba离子后，在96%的AMs和70%的ICMs中出现了被硝苯地平阻断的延长的SRCs。在施加10 mM Ba溶液时，AMs和ICMs的SRCs持续时间无显著差异（分别为2.8秒和2.0秒）。在含有10 mM Ba溶液的培养基中，AMs和ICMs的静息膜电位（RMPs）分别降至78%和74%，且在含有10 mM Ba的培养基中AMs的输入电阻（Rin）增加到161%。在AMs和ICMs中均观察到缓慢的超极化后电位，且在施加Ba期间完全消失。