Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Cancer Sci. 2013 May;104(5):531-5. doi: 10.1111/cas.12119. Epub 2013 Mar 31.
Pancreatic cancer is an aggressive cancer with poor prognosis. Little is known about the immune response in the tumor microenvironment after chemotherapy for initially unresectable tumor. The purpose of this study was to investigate the immunological effects of chemoradiation therapy in the tumor microenvironment of pancreatic adenocarcinoma. Seventeen patients with pancreatic adenocarcinoma with and without preoperative chemoradiation therapy were retrospectively analyzed using immunohistochemical methods for HLA class I heavy chain, CD4(+), CD8(+), CD45RO(+) and Foxp3(+) T cell infiltrations. Seven of the 17 study patients received preoperative chemoradiation therapy. There were no statistically significant differences in the number of CD4(+) and CD8(+) T cell infiltrations in the tumor microenvironment. However, the number of Foxp3(+) T cell infiltrations was significantly lower in the neoadjuvant chemoradiation therapy group. The HLA class I expression status was the same between the two groups. In conclusion, preoperative chemoradiation therapy in pancreatic adenocarcinoma is useful for reducing regulatory T cell levels in combination with its direct cytotoxic effects.
胰腺癌是一种侵袭性强、预后差的癌症。对于初始不可切除肿瘤化疗后肿瘤微环境中的免疫反应，人们知之甚少。本研究旨在探讨化疗放疗对胰腺腺癌肿瘤微环境的免疫学影响。使用免疫组织化学方法对 17 例接受和未接受术前放化疗的胰腺腺癌患者进行回顾性分析，检测 HLA Ⅰ类重链、CD4(+)、CD8(+)、CD45RO(+)和 Foxp3(+)T 细胞浸润。17 例研究患者中有 7 例接受了术前放化疗。肿瘤微环境中 CD4(+)和 CD8(+)T 细胞浸润的数量没有统计学差异。然而，新辅助放化疗组 Foxp3(+)T 细胞浸润的数量明显较低。两组的 HLA Ⅰ类表达状态相同。总之，术前放化疗对胰腺腺癌是有用的，可结合其直接细胞毒性作用降低调节性 T 细胞水平。
