Xu Chao-ping, Cai Hui-li, He Li, Ma Zi, Liu Shang-qin
Department of Hematology, Wuhan University School of Medicine, Wuhan, China.
Zhonghua Xue Ye Xue Za Zhi. 2012 Nov;33(11):938-43.
To investigate the effect of baicalein on proliferation and migration of multiple myeloma (MM) cell lines and its molecular mechanism.
The MM cell line RPMI-8226 and U266 cells were used as the model, and treated with different concentration and time of baicalein the effect of baicalein on the MM cells proliferation was assessed by MTT assay. With or without baicalein or Interleukin-6 (IL-6) treatment, the β-catenin protein level was analyzed by immunofluorescence assay and western blot assay and mRNA levels of β-catenin, c-myc, cyclin D1 and integrin 7 gene by RT-PCR. Transwell chamber migration assay was used to detect the cells migration ability with different concentration of baicalein cultured.
Baicalein inhibited the MM cell line RPMI 8226 and U266 cell proliferation in a dose- and time-dependent manner. It simultaneously inhibited β-catenin protein level to resist the effect of IL-6 on inducing MM cell proliferation, and resulted in decrease of β-catenin, c-myc, cyclinD1 and integrin β7 mRNA levels. Baicalein also decreased migration ability of MM cells in a dose-dependent manner by SDF-1.
Baicalein can inhibit MM cells proliferation and migration, and its molecular mechanisms are associated with inhibition of proliferation related genes β-catenin, c-myc, cyclin D1 and integrin β7 expression.
探讨黄芩苷对多发性骨髓瘤（MM）细胞系增殖和迁移的影响及其分子机制。
以MM细胞系RPMI-8226和U266细胞为模型，用不同浓度和时间的黄芩苷处理，采用MTT法评估黄芩苷对MM细胞增殖的影响。在有或无黄芩苷或白细胞介素-6（IL-6）处理的情况下，通过免疫荧光法和蛋白质印迹法分析β-连环蛋白的蛋白水平，通过逆转录聚合酶链反应（RT-PCR）检测β-连环蛋白、c-myc、细胞周期蛋白D1和整合素β7基因的mRNA水平。采用Transwell小室迁移试验检测不同浓度黄芩苷培养下细胞的迁移能力。
黄芩苷以剂量和时间依赖性方式抑制MM细胞系RPMI 8226和U266细胞的增殖。它同时抑制β-连环蛋白的蛋白水平以抵抗IL-6对诱导MM细胞增殖的作用，并导致β-连环蛋白、c-myc、细胞周期蛋白D1和整合素β7的mRNA水平降低。黄芩苷还以剂量依赖性方式降低MM细胞在基质细胞衍生因子-1（SDF-1）作用下的迁移能力。
黄芩苷可抑制MM细胞的增殖和迁移，其分子机制与抑制增殖相关基因β-连环蛋白、c-myc、细胞周期蛋白D1和整合素β7的表达有关。
