Fox K R, Kentebe E
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, UK.
Nucleic Acids Res. 1990 Apr 25;18(8):1957-63. doi: 10.1093/nar/18.8.1957.
DNA fragments containing the sequence CG(AT)nCG have been used in footprinting experiments to assess the effect of echinomycin, which binds to CG steps, on the structure of the central AT region. DNAase I normally cuts ApT much better than TpA; in the presence of the drug this preference is retained but cleavage at TpA is enhanced. Changes in cleavage by micrococcal nuclease have also been observed. Echinomycin renders alternate adenines hyperreactive to diethylpyrocarbonate. The results suggest that echinomycin induces structural changes in regions surrounding its binding site and that these can be cooperatively propagated over several turns of the DNA helix.
含有序列CG(AT)nCG的DNA片段已用于足迹实验，以评估与CG步结合的放线菌素对中央AT区域结构的影响。DNA酶I通常切割ApT的效果比切割TpA好得多；在药物存在的情况下，这种偏好得以保留，但TpA处的切割得到增强。也观察到了微球菌核酸酶切割的变化。放线菌素使交替的腺嘌呤对焦碳酸二乙酯高度敏感。结果表明，放线菌素在其结合位点周围的区域诱导结构变化，并且这些变化可以在DNA螺旋的几圈中协同传播。
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