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棘霉素与序列CG(AT)nCG结合会改变中央AT区域的结构。

Echinomycin binding to the sequence CG(AT)nCG alters the structure of the central AT region.

作者信息

Fox K R, Kentebe E

机构信息

Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, UK.

出版信息

Nucleic Acids Res. 1990 Apr 25;18(8):1957-63. doi: 10.1093/nar/18.8.1957.

DOI:10.1093/nar/18.8.1957
PMID:2336384
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330668/
Abstract

DNA fragments containing the sequence CG(AT)nCG have been used in footprinting experiments to assess the effect of echinomycin, which binds to CG steps, on the structure of the central AT region. DNAase I normally cuts ApT much better than TpA; in the presence of the drug this preference is retained but cleavage at TpA is enhanced. Changes in cleavage by micrococcal nuclease have also been observed. Echinomycin renders alternate adenines hyperreactive to diethylpyrocarbonate. The results suggest that echinomycin induces structural changes in regions surrounding its binding site and that these can be cooperatively propagated over several turns of the DNA helix.

摘要

含有序列CG(AT)nCG的DNA片段已用于足迹实验，以评估与CG步结合的放线菌素对中央AT区域结构的影响。DNA酶I通常切割ApT的效果比切割TpA好得多；在药物存在的情况下，这种偏好得以保留，但TpA处的切割得到增强。也观察到了微球菌核酸酶切割的变化。放线菌素使交替的腺嘌呤对焦碳酸二乙酯高度敏感。结果表明，放线菌素在其结合位点周围的区域诱导结构变化，并且这些变化可以在DNA螺旋的几圈中协同传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a0/330668/7e850fc648ab/nar00192-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a0/330668/072cb350fc90/nar00192-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a0/330668/7e850fc648ab/nar00192-0028-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a0/330668/072cb350fc90/nar00192-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0a0/330668/7e850fc648ab/nar00192-0028-a.jpg

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1
Echinomycin binding to the sequence CG(AT)nCG alters the structure of the central AT region.棘霉素与序列CG(AT)nCG结合会改变中央AT区域的结构。
Nucleic Acids Res. 1990 Apr 25;18(8):1957-63. doi: 10.1093/nar/18.8.1957.
2
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Chemical probes reveal no evidence of Hoogsteen base pairing in complexes formed between echinomycin and DNA in solution.化学探针未显示在溶液中放线菌素与DNA形成的复合物中有Hoogsteen碱基配对的证据。
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Sequence-specific binding of [N-MeCys3,N-MeCys7]TANDEM to TpA.[N-甲基半胱氨酸3，N-甲基半胱氨酸7]串联体与TpA的序列特异性结合。
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Indirect readout in drug-DNA recognition: role of sequence-dependent DNA conformation.药物与DNA识别中的间接读出：序列依赖性DNA构象的作用

本文引用的文献

1
The molecular structure of a DNA-triostin A complex.一种DNA-三奥菌素A复合物的分子结构。
Science. 1984 Sep 14;225(4667):1115-21. doi: 10.1126/science.6474168.
2
DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting.通过DNA酶I足迹法揭示的放线菌素和偏端霉素产生的DNA结构变异。
Nucleic Acids Res. 1984 Dec 21;12(24):9271-85. doi: 10.1093/nar/12.24.9271.
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Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequences.放线菌素与DNA的序列特异性结合：影响侧翼序列构象变化的证据。
Nucleic Acids Res. 2008 Feb;36(2):376-86. doi: 10.1093/nar/gkm892. Epub 2007 Nov 26.
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Infrared linear dichroism studies of DNA-drug complexes: quantitative determination of the drug-induced restriction of the B-A transition.DNA-药物复合物的红外线性二色性研究：药物诱导的B-A转变限制的定量测定
Nucleic Acids Res. 1994 Mar 11;22(5):787-91. doi: 10.1093/nar/22.5.787.
6
Echinomycin binding to alternating AT.放线菌素与交替的AT序列结合。
Nucleic Acids Res. 1991 Dec 25;19(24):6725-30. doi: 10.1093/nar/19.24.6725.
7
Interaction of echinomycin with An.Tn. and (AT)n regions flanking its CG binding site.
Nucleic Acids Res. 1991 Dec 25;19(24):6719-24. doi: 10.1093/nar/19.24.6719.
8
Proton NMR study of the [d(ACGTATACGT)]2-2echinomycin complex: conformational changes between echinomycin binding sites.[二（ACGTATACGT）]-2棘霉素复合物的质子核磁共振研究：棘霉素结合位点之间的构象变化
Nucleic Acids Res. 1992 May 25;20(10):2411-20. doi: 10.1093/nar/20.10.2411.
Nucleic Acids Res. 1984 Jun 25;12(12):4865-79. doi: 10.1093/nar/12.12.4865.
4
Echinomycin binding sites on DNA.放线菌素与DNA上的结合位点。
Science. 1984 Sep 14;225(4667):1122-7. doi: 10.1126/science.6089341.
5
Echinomycin: a bifunctional intercalating antibiotic.棘霉素：一种双功能嵌入型抗生素。
Nature. 1974 Dec 20;252(5485):653-7. doi: 10.1038/252653a0.
6
A comparison of the structure of echinomycin and triostin A complexed to a DNA fragment.棘霉素和曲奥菌素A与一个DNA片段复合后的结构比较。
Nucleic Acids Res. 1985 Apr 11;13(7):2305-23. doi: 10.1093/nar/13.7.2305.
7
Actinomycin D facilitates transition of AT domains in molecules of sequence (AT)nAGCT(AT)n to a DNAse I detectable alternating structure.放线菌素D促进序列为(AT)nAGCT(AT)n的分子中AT结构域向DNA酶I可检测的交替结构转变。
Nucleic Acids Res. 1987 Jan 26;15(2):839-52. doi: 10.1093/nar/15.2.839.
8
(A-T)n tracts embedded in random sequence DNA--formation of a structure which is chemically reactive and torsionally deformable.嵌入随机序列DNA中的（A-T）n序列——形成一种具有化学反应性和可扭转变形的结构。
Nucleic Acids Res. 1986 Dec 9;14(23):9291-309. doi: 10.1093/nar/14.23.9291.
9
The use of micrococcal nuclease as a probe for drug-binding sites on DNA.使用微球菌核酸酶作为DNA上药物结合位点的探针。
Biochim Biophys Acta. 1987 Jul 14;909(2):145-55. doi: 10.1016/0167-4781(87)90036-4.
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NMR studies of echinomycin bisintercalation complexes with d(A1-C2-G3-T4) and d(T1-C2-G3-A4) duplexes in aqueous solution: sequence-dependent formation of Hoogsteen A1.T4 and Watson--Crick T1.A4 base pairs flanking the bisintercalation site.水溶液中棘霉素与d(A1-C2-G3-T4)和d(T1-C2-G3-A4)双链体双插入复合物的核磁共振研究：双插入位点两侧Hoogsteen A1.T4和沃森-克里克T1.A4碱基对的序列依赖性形成。
Biochemistry. 1988 Mar 8;27(5):1744-51. doi: 10.1021/bi00405a054.