[N-甲基半胱氨酸3，N-甲基半胱氨酸7]串联体与TpA的序列特异性结合。

Sequence-specific binding of [N-MeCys3,N-MeCys7]TANDEM to TpA.

作者信息

Lavesa M, Olsen R K, Fox K R

机构信息

Department of Physiology and Pharmacology, University of Southampton, U.K.

出版信息

Biochem J. 1993 Jan 15;289 ( Pt 2)(Pt 2):605-7. doi: 10.1042/bj2890605.

DOI:10.1042/bj2890605

PMID:8424802

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132212/

Abstract

The sequence selective binding of [N-MeCys3,N-MeCys7]TANDEM to DNA has been studied by footprinting experiments on DNA fragments containing the self-complementary sequences CGCGATATCGCG, CGCGTATACGCG, CGCGTTAACGCG and CGCGAATTCGCG. DNAase I and micrococcal nuclease reveal drug-induced footprints with the central sequences ATAT, TATA and TTAA, but not AATT, suggesting that the ligand binds to the dinucleotide TpA. The ligand renders certain adenines hyper-reactive to diethyl pyrocarbonate. These are observed with ATAT, TATA and TTAA, but not AATT, and are located both within, and distal to, the TpA-binding sites.

摘要

通过对含有自我互补序列CGCGATATCGCG、CGCGTATACGCG、CGCGTTAACGCG和CGCGAATTCGCG的DNA片段进行足迹实验，研究了[N-MeCys3,N-MeCys7]TANDEM与DNA的序列选择性结合。DNA酶I和微球菌核酸酶揭示了药物诱导的足迹，其中心序列为ATAT、TATA和TTAA，但不是AATT，这表明配体与二核苷酸TpA结合。该配体使某些腺嘌呤对焦碳酸二乙酯具有高反应性。在ATAT、TATA和TTAA中观察到这种情况，但在AATT中未观察到，并且它们位于TpA结合位点内及其远端。