Yang Ning-ning, He Kui-fang, Pan Yi-huai, Wang Hui-ning, Deng Hui, Ma Jian-feng
Department of Oral Medicine, School of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.
Shanghai Kou Qiang Yi Xue. 2012 Dec;21(6):637-42.
An anticaries DNA vaccine pEGFP-N1-Srv+ was used to immunize rats by different immune pathways. The expression of recombinant plasmid in different tissues in vivo and the specific immune response and protection effects against dental caries were observed.
20 SD rats were divided into 4 groups, immunized with the recombinant plasmid pEGFP-N1-SrV+ by submandibular gland-target injection(TSG), and intramuscular injection,respectively; then the expression of recombinant plasmid in different tissues were detected by immunohistochemistry technique. 24 SD rats were divided into 4 groups, immunized with recombinant plasmid pEGFP-N1-SrV+ of 100 μg, then boosted once after two weeks, through the same routes as above;then indirect ELISA technique was used to detect the specific antibodies. Keyes caries scores were used to evaluate the anticaries effects. The data was analyzed by using SPSS17.0 software package.
(1)Recombinant plasmid was positively expressed in the muscle fibers and submandibular glands.(2)The specific salivary anti-SR IgA and serum IgG were detected, and the peak time of the antibodies level appeared 4 weeks after initial . At the 4th week, the levels of specific anti-SR antibodies were higher in the experimental group than that in the negative control group. The levels of salivary specific anti-SR IgA were significantly higher in TSG immunization group than that in the intramuscular injection group. (3)Keyes caries scores were not significantly different between the experimental groups and negative control groups. recombinant plasmid pEGFP-N1-Srv+ expressed in vivo and effectively increased specific salivary anti-SR IgA and serum IgG, and TSG immunization route significantly increased the specific salivary anti-SR IgA compared with the intramuscular immunization route;however, the recombinant plasmid pEGFP-N1-Srv+ alone can not protect the rats from dental caries.
The recombinant plasmid pEGFP-N1-SrV+ expressed in vivo and effectively increased specific salivary anti-SR IgA and serum IgC, and TSG immunization route significantly increased the specific salivary anti-SR IgA compared with the intramuscular immunization route; however, the recombinant plasmid pEGFP-N1-Srv+ alone can not protect the rats from dental caries.
采用抗龋DNA疫苗pEGFP-N1-Srv+通过不同免疫途径免疫大鼠，观察重组质粒在体内不同组织中的表达情况以及对龋齿的特异性免疫应答和保护作用。
将20只SD大鼠分为4组，分别通过下颌下腺靶向注射（TSG）和肌肉注射用重组质粒pEGFP-N1-SrV+进行免疫；然后采用免疫组织化学技术检测重组质粒在不同组织中的表达。将24只SD大鼠分为4组，用100μg重组质粒pEGFP-N1-SrV+进行免疫，两周后通过上述相同途径进行一次加强免疫；然后采用间接ELISA技术检测特异性抗体。用Keyes龋齿评分评估抗龋效果。数据采用SPSS17.0软件包进行分析。
（1）重组质粒在肌纤维和下颌下腺中呈阳性表达。（2）检测到唾液特异性抗SR IgA和血清IgG，抗体水平峰值出现在初次免疫后4周。在第4周时，实验组特异性抗SR抗体水平高于阴性对照组。TSG免疫组唾液特异性抗SR IgA水平显著高于肌肉注射组。（3）实验组与阴性对照组之间的Keyes龋齿评分无显著差异。重组质粒pEGFP-N1-Srv+在体内表达并有效增加了唾液特异性抗SR IgA和血清IgG，与肌肉免疫途径相比，TSG免疫途径显著增加了唾液特异性抗SR IgA；然而，单独的重组质粒pEGFP-N1-Srv+不能保护大鼠免受龋齿侵害。
重组质粒pEGFP-N1-SrV+在体内表达并有效增加了唾液特异性抗SR IgA和血清IgC，与肌肉免疫途径相比，TSG免疫途径显著增加了唾液特异性抗SR IgA；然而，单独的重组质粒pEGFP-N1-Srv+不能保护大鼠免受龋齿侵害。
