Ma Jinling, van den Beucken Jeroen J J P, Both Sanne K, Prins Henk-Jan, Helder Marco N, Yang Fang, Jansen John A
Department of Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands.
Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
J Tissue Eng Regen Med. 2015 Jul;9(7):779-88. doi: 10.1002/term.1704. Epub 2013 Jan 31.
Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived mesenchymal stem cells (AT-MSCs) are the most frequently used stem cells in tissue engineering. Due to major clinical demands, it is necessary to find an optimally safe and efficient way for large-scale expansion of these cells. Considering the nutritional source in the culture medium and method, this study aimed to analyze the effects of FBS- and PL-supplemented media on osteogenesis in stem cell mono- and co-cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell metabolic activity and proliferation increased in PL- compared to FBS-supplemented media in mono- and co-cultures for both BM-MSCs and AT-MSCs. In addition, calcium deposition was cell type dependent and decreased for BM-MSCs but increased for AT-MSCs in PL-supplemented medium in both mono- and co-cultures. Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed similar results compared to AT-MSCs monocultures.
人骨髓间充质干细胞（BM-MSCs）和人脂肪组织间充质干细胞（AT-MSCs）是组织工程中最常用的干细胞。由于临床需求巨大，有必要找到一种最优的安全且高效的方法来大规模扩增这些细胞。考虑到培养基中的营养来源和方法，本研究旨在分析添加胎牛血清（FBS）和血小板裂解物（PL）的培养基对干细胞与人类脐静脉内皮细胞（HUVECs）单培养和共培养时成骨作用的影响。结果表明，对于BM-MSCs和AT-MSCs，在单培养和共培养中，与添加FBS的培养基相比，添加PL的培养基中细胞代谢活性和增殖均增加。此外，钙沉积取决于细胞类型，在单培养和共培养中，添加PL的培养基中BM-MSCs的钙沉积减少，而AT-MSCs的钙沉积增加。基于共培养的效果，在添加FBS和PL的培养基中，与BM-MSCs单培养相比，BM-MSCs/HUVECs共培养增强了成骨作用，而与AT-MSCs单培养相比，AT-MSCs/HUVECs共培养显示出相似的结果。
