Zellner Phillip A, Sahari Ali, Hosseini Yahya, Behkam Bahareh, Agah Masoud
VT MEMS Lab, Virginia Tech, VA 24060, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:6285-8. doi: 10.1109/EMBC.2012.6347431.
We present the development of a batch trapping, insulator-based dielectrophoretic (iDEP) device with three-dimensional design. The microfluidic devices use DC-biased, AC electric fields to selectively manipulate biological particles based on their electric properties. The mold for the polymer microdevices is fabricated using an RIE-lag technique which creates microchannels with varying depths using a single etch process. The resulting three-dimensional insulating constrictions permit operation at low applied voltages. By varying both the applied frequency and the ratio of AC to DC electric fields, the iDEP device can trap and separate polystyrene beads and E. coli cells.
我们展示了一种具有三维设计的基于绝缘体的介电电泳（iDEP）批量捕获装置的开发。微流控装置利用直流偏置的交流电场，根据生物颗粒的电学性质对其进行选择性操控。聚合物微器件的模具采用反应离子刻蚀滞后（RIE-lag）技术制造，该技术通过单次蚀刻工艺创建具有不同深度的微通道。由此产生的三维绝缘收缩结构允许在低施加电压下运行。通过改变施加频率以及交流与直流电场的比例，iDEP装置可以捕获和分离聚苯乙烯珠和大肠杆菌细胞。
