Field J B, Bloom G, Chou M C, Kerins M E, Larsen P R, Kotani M, Kariya T, Dekker A
J Clin Endocrinol Metab. 1978 Nov;47(5):1052-8. doi: 10.1210/jcem-47-5-1052.
Effects of TSH on the adenylate cyclase-cAMP system and some parameters of intermediary metabolism were investigated in human thyroid carcinoma and adjacent normal thyroid tissue. Basal adenylate cyclase activity and cAMP concentrations were significantly higher in carcinomatous tissue. Basal [1-14C]glucose oxidation, 32Pi incorporation into phospholipids, and organification of iodide were similar in both tissues. Stimulation of cAMP by TSH was significantly greater in normal compared to carcinomatous tissue. In neither tissue was there a good correlation between TSH stimulation of adenylate cyclase activity and cAMP concentrations. The TSH stimulation of 32Pi incorporation into phospholipids by TSH was significantly greater in normal tissue. The mean effect of TSH on iodide organification and glucose oxidation was similar in normal and carcinomatous tissue. Although specific binding of TSH was demonstrated in both normal and carcinomatous tissue, it did not correlate very well with stimulation of adenylate cyclase activity. Hormones other than TSH also augmented adenylate cyclase activity in two of the carcinomas. In individual patients, the relative responsivity of carcinomatous tissue compared to normal was not always consistent when all of the metabolic parameters were considered.
在人甲状腺癌及相邻正常甲状腺组织中，研究了促甲状腺激素（TSH）对腺苷酸环化酶 - cAMP系统及中间代谢的一些参数的影响。癌组织中的基础腺苷酸环化酶活性和cAMP浓度显著更高。两种组织中的基础[1-14C]葡萄糖氧化、32Pi掺入磷脂以及碘化物的有机化作用相似。与癌组织相比，正常组织中TSH对cAMP的刺激作用显著更大。在两种组织中，TSH刺激腺苷酸环化酶活性与cAMP浓度之间均无良好的相关性。TSH对正常组织中32Pi掺入磷脂的刺激作用显著更大。TSH对碘化物有机化作用和葡萄糖氧化的平均效应在正常组织和癌组织中相似。尽管在正常组织和癌组织中均证实了TSH的特异性结合，但它与腺苷酸环化酶活性的刺激作用相关性不太好。除TSH外的其他激素也增强了其中两个癌组织中的腺苷酸环化酶活性。在个体患者中，当考虑所有代谢参数时，癌组织与正常组织相比的相对反应性并不总是一致的。
