使用免疫组织化学法比较人边缘龈和龈乳头中纤连蛋白的含量

Comparison of fibronectin in human marginal gingiva and interdental papilla using immunohistochemistry.

作者信息

Tavakoli Mohammad, Bateni Ensiyeh, Attarbashi-Moghadam Fazeleh, Talebi Ardeshir, Yaghini Jaber, Mogharehabed Ahmad

机构信息

Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Dent Res J (Isfahan). 2011 Dec;8(Suppl 1):S109-13.

PMID:23372588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3556296/

Abstract

BACKGROUND

The differences between marginal gingiva and interdental papilla may be due to variation in the molecular composition of these two different anatomical structures. The aim of this study was to evaluate the staining intensity of fibronectin in human marginal gingiva and interdental papilla.

MATERIALS AND METHODS

In a prospective analytical study, 16 healthy subjects needing crown lengthening surgery were selected. All participants were medically healthy, non-smokers, with no medication intake, and a healthy periodontium. During surgery, facial/buccal marginal gingiva and interdental papilla were separately harvested. The specimens were subjected to hematoxylin and eosin, histochemical (Masson' strichorom, reticulin, and elastic), and immunohistochemical staining for evaluation of morphology and inflammation; assessment of connective tissue fibers (collagen, reticulin, and elastic); and determination of fibronectin staining intensity. The data were analyzed by Spsssoftware, Wilcoxon, and Spearman tests. P<0.05 was considered to be statistically significant.

RESULTS

From a total of 32 specimens, 21 specimens were found to be normal or having mild inflammation, while the remaining specimens had moderate to severe inflammation in some parts. Collagen fibers were found to be dense in reticular connective tissue and degenerated in the region of inflammation. Reticulin fibers strongly stained near epithelium. Elastic fibers were sparsely found. Mean fibronectin staining intensity between marginal gingiva and interdental papilla was not statically significant (P=0.44). There is no statistically significant correlation between tissue inflammation and fibronectin staining intensity (P=0.76 for marginal gingival and P=0.20 for interdental papilla). Considering all specimens, fibronectin staining intensity of connective tissue adjacent to Sulcular/Junctional epithelium was higher than reticular connective tissue (P=0.003) and higher than connective tissue adjacent to oral epithelium (P<0.001).

CONCLUSION

This study did not show any difference in interdental papilla and marginal gingival with respect to fibronectin composition. More studies in this context are needed.

摘要

背景

边缘龈与龈乳头之间的差异可能归因于这两种不同解剖结构分子组成的变化。本研究的目的是评估纤连蛋白在人边缘龈和龈乳头中的染色强度。

材料与方法

在一项前瞻性分析研究中，选取了16名需要进行牙冠延长术的健康受试者。所有参与者身体健康，不吸烟，未服用药物，且牙周健康。手术过程中，分别采集面部/颊侧边缘龈和龈乳头。对标本进行苏木精-伊红染色、组织化学染色（马松三色染色、网状纤维染色和弹性纤维染色）以及免疫组织化学染色，以评估形态学和炎症情况；评估结缔组织纤维（胶原纤维、网状纤维和弹性纤维）；并确定纤连蛋白的染色强度。数据采用Spss软件、威尔科克森检验和斯皮尔曼检验进行分析。P<0.05被认为具有统计学意义。

结果

在总共32个标本中，21个标本被发现正常或有轻度炎症，而其余标本在某些部位有中度至重度炎症。发现胶原纤维在网状结缔组织中密集，在炎症区域退化。网状纤维在上皮附近强烈染色。弹性纤维稀少。边缘龈和龈乳头之间的平均纤连蛋白染色强度无统计学意义（P=0.44）。组织炎症与纤连蛋白染色强度之间无统计学显著相关性（边缘龈P=0.76，龈乳头P=0.20）。考虑所有标本，龈沟/结合上皮相邻结缔组织的纤连蛋白染色强度高于网状结缔组织（P=0.003），且高于口腔上皮相邻结缔组织（P<0.001）。