骨髓来源的内皮祖细胞促进造血干细胞移植后的造血重建。
Bone marrow-derived endothelial progenitor cells promote hematopoietic reconstitution after hematopoietic stem cell transplantation.
作者信息
Yan Z, Zeng L, Li Z, Zhang H, Chen W, Jia L, Chen C, Cheng H, Cao J, Xu K
机构信息
Department of Haematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
出版信息
Transplant Proc. 2013 Jan-Feb;45(1):427-33. doi: 10.1016/j.transproceed.2012.03.064.
OBJECTIVE
A hematopoietic deficit is a serious complication after hematopoietic stem cell transplantation. It has been shown that fetal blood-derived endothelial progenitor cells (EPCs) can promote hematopoietic reconstitution after transplantation. This study investigated whether EPCs from bone marrow (BM) of adult mice could promote hematopoietic reconstitution.
METHODS
Lethally irradiated BALB/c mice were administered BM cells or BM cells plus EPCs.
RESULTS
The results showed that EPC-treated mice displayed accelerated recovery of peripheral blood white blood cells and reticulocytes. But the platelets were not significantly different with versus without EPCs. Accelerated recovery of BM sinusoidal vessels, promotion of stem cell implantation, and decreased adipocyte formation were associated with the mechanism. Systemic administration of anti-vascular endothelial cadherin antibody neutralized these effects significantly.
CONCLUSION
These data showed that BM-derived EPC infusions augmented hematopoiesis suggesting a new approach to promote hematopoiesis.
目的
造血功能缺陷是造血干细胞移植后的一种严重并发症。研究表明，胎儿血源性内皮祖细胞（EPCs）可促进移植后的造血重建。本研究调查了成年小鼠骨髓（BM）来源的EPCs是否能促进造血重建。
方法
对经致死剂量照射的BALB/c小鼠给予骨髓细胞或骨髓细胞加EPCs。
结果
结果显示，接受EPC治疗的小鼠外周血白细胞和网织红细胞恢复加速。但血小板在有或无EPCs的情况下无显著差异。骨髓窦状血管的加速恢复、干细胞植入的促进以及脂肪细胞形成的减少与该机制有关。全身给予抗血管内皮钙黏蛋白抗体可显著中和这些作用。
结论
这些数据表明，骨髓来源的EPC输注可增强造血功能，提示了一种促进造血的新方法。
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