Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Res Vet Sci. 2013 Jun;94(3):545-8. doi: 10.1016/j.rvsc.2013.01.007. Epub 2013 Jan 31.
Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a γ-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for γ-H2AX.
猫科动物注射部位肉瘤（ISS）是一种高度侵袭性的软组织肿瘤，通常采用放射治疗。细胞脱氧核糖核酸（DNA）是辐射生物效应的主要靶点，细胞杀伤与双链 DNA 断裂（DSBs）的数量相关。本研究的目的是确定是否可以使用市售的 DNA DSB 检测试剂盒检测到猫科动物 ISS 细胞的辐射损伤。用γ-H2AX 化学发光试剂盒检测辐照猫科动物 ISS 细胞的 DSB 诱导程度；通过 Western Blot 分析验证结果。与对照细胞相比，辐照细胞的双链断裂诱导明显增加，Western Blot 分析支持这一结果。使用市售的小鼠抗人单克隆抗体γ-H2AX 可以间接检测单次照射后猫科肉瘤细胞的 DNA 损伤。
