处理微污染原水的移动床生物膜反应器中温度和 NH4(+)-N 负荷变化对生物膜特性的响应。
Responses of biofilm characteristics to variations in temperature and NH4(+)-N loading in a moving-bed biofilm reactor treating micro-polluted raw water.
机构信息
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
出版信息
Bioresour Technol. 2013 Mar;131:365-73. doi: 10.1016/j.biortech.2012.12.172. Epub 2013 Jan 4.
A pilot-scale moving-bed biofilm reactor (MBBR) for biological treatment of micro-polluted raw water was operated over 400days to investigate the responses of biofilm characteristics and nitrification performance to variations in temperature and NH4(+)-N loading. The mean removal efficiency of NH4(+)-N in the MBBR reached 71.4±26.9%, and batch experiments were performed to study nitrification kinetics for better process understanding. Seven physical-chemical parameters, including volatile solids (VS), polysaccharides (PS) and phospholipids (PL) increased firstly, and then rapidly decreased with increasing temperature and NH4(+)-N loading, and properly characterized the attached biomass during biofilm development and detachment in the MBBR. The biofilm compositions were described by six ratios, e.g., PS/VS and PL/VS ratios showed different variation trends, indicating different responses of PS and PL to the changes in temperature and NH4(+)-N loading. Furthermore, fluorescent in situ hybridization (FISH) analysis revealed that increased NH4(+)-N loadings caused an enrichment of the nitrifying biofilm.
采用中试规模移动床生物膜反应器（MBBR）对微污染原水进行生物处理，运行 400 余天，考察了温度和 NH4(+)-N 负荷变化对生物膜特性和硝化性能的响应。MBBR 对 NH4(+)-N 的平均去除效率达到 71.4±26.9%，并进行了批式实验以研究硝化动力学，从而更好地理解工艺。为了更好地描述生物膜的发展和脱落过程，在 MBBR 中，有 7 个物理化学参数（包括挥发性固体（VS）、多糖（PS）和磷脂（PL））首先增加，然后随着温度和 NH4(+)-N 负荷的增加而迅速减少。生物膜组成可以用 6 个比值来描述，例如 PS/VS 和 PL/VS 比值呈现出不同的变化趋势，表明 PS 和 PL 对温度和 NH4(+)-N 负荷变化的响应不同。此外，荧光原位杂交（FISH）分析表明，增加的 NH4(+)-N 负荷导致硝化生物膜的富集。
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