State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
Bioresour Technol. 2013 Mar;131:365-73. doi: 10.1016/j.biortech.2012.12.172. Epub 2013 Jan 4.
A pilot-scale moving-bed biofilm reactor (MBBR) for biological treatment of micro-polluted raw water was operated over 400days to investigate the responses of biofilm characteristics and nitrification performance to variations in temperature and NH4(+)-N loading. The mean removal efficiency of NH4(+)-N in the MBBR reached 71.4±26.9%, and batch experiments were performed to study nitrification kinetics for better process understanding. Seven physical-chemical parameters, including volatile solids (VS), polysaccharides (PS) and phospholipids (PL) increased firstly, and then rapidly decreased with increasing temperature and NH4(+)-N loading, and properly characterized the attached biomass during biofilm development and detachment in the MBBR. The biofilm compositions were described by six ratios, e.g., PS/VS and PL/VS ratios showed different variation trends, indicating different responses of PS and PL to the changes in temperature and NH4(+)-N loading. Furthermore, fluorescent in situ hybridization (FISH) analysis revealed that increased NH4(+)-N loadings caused an enrichment of the nitrifying biofilm.
采用中试规模移动床生物膜反应器（MBBR）对微污染原水进行生物处理，运行 400 余天，考察了温度和 NH4(+)-N 负荷变化对生物膜特性和硝化性能的响应。MBBR 对 NH4(+)-N 的平均去除效率达到 71.4±26.9%，并进行了批式实验以研究硝化动力学，从而更好地理解工艺。为了更好地描述生物膜的发展和脱落过程，在 MBBR 中，有 7 个物理化学参数（包括挥发性固体（VS）、多糖（PS）和磷脂（PL））首先增加，然后随着温度和 NH4(+)-N 负荷的增加而迅速减少。生物膜组成可以用 6 个比值来描述，例如 PS/VS 和 PL/VS 比值呈现出不同的变化趋势，表明 PS 和 PL 对温度和 NH4(+)-N 负荷变化的响应不同。此外，荧光原位杂交（FISH）分析表明，增加的 NH4(+)-N 负荷导致硝化生物膜的富集。
