Department of Human Anatomy, Nanjing Medical University, Nanjing, China.
Neuroscience. 2013 Apr 16;236:262-70. doi: 10.1016/j.neuroscience.2013.01.039. Epub 2013 Jan 30.
Medial prefrontal cortex (mPFC) has been implicated in the reward process and the development of cocaine addiction. In the current study, we used a proteomics-based approach, combining two-dimensional gel electrophoresis (2-DE) with mass spectrometry (MS), to analyze protein expression profiles of rat mPFC after repeated cocaine exposure. Conditioned place preference (CPP) assay was used here to evaluate cocaine-induced reward effect in rats. We detected about 3100 protein spots in rat mPFC. After repeated cocaine exposure, 125 spots were changed by more than 1.1-fold of control levels. Among them, 71 spots with 1.5-fold or greater changes in protein expression over control levels have been identified, including 50 spots that were up-regulated and 21 spots that were down-regulated by repeated cocaine exposure. These identified proteins that showed significant changes in expression in mPFC after repeated cocaine exposure may be useful biomarkers for assessing cocaine abuse and potential new targets for investigating the mechanism of cocaine abuse.
内侧前额叶皮层（mPFC）参与了奖励过程和可卡因成瘾的发展。在本研究中，我们使用了一种基于蛋白质组学的方法，结合二维凝胶电泳（2-DE）和质谱（MS），来分析重复可卡因暴露后大鼠 mPFC 的蛋白质表达谱。条件性位置偏爱（CPP）试验用于评估大鼠的可卡因诱导的奖励效应。我们在大鼠 mPFC 中检测到约 3100 个蛋白点。在重复可卡因暴露后，有 125 个点的表达水平变化超过对照水平的 1.1 倍。其中，有 71 个蛋白点的表达水平变化超过对照水平的 1.5 倍，包括 50 个上调和 21 个下调的蛋白点。这些在重复可卡因暴露后 mPFC 中表达显著变化的鉴定蛋白可能是评估可卡因滥用的有用生物标志物，也是研究可卡因滥用机制的潜在新靶点。
