Zweckmair Thomas, Böhmdorfer Stefan, Bogolitsyna Anna, Rosenau Thomas, Potthast Antje, Novalin Senad
1University of Natural Resources and Life Sciences (BOKU Vienna), Muthgasse 18, A-1190 Vienna, Austria.
J Chromatogr Sci. 2014 Feb;52(2):169-75. doi: 10.1093/chromsci/bmt004. Epub 2013 Feb 1.
This study presents a liquid chromatography-ultraviolet (LC-UV) method for the accurate analysis of formose reaction products with regard to sum parameters. Limitations encountered in gas chromatography (e.g., thermal stability of analytes) were overcome. First, alditols are O-benzoylated and subjected to reversed-phase chromatography. They are then detected by UV and by electrospray ionization-mass spectrometry (ESI-MS) for validation purposes. Compared with standard 2,4-dinitrophenylhydrazine labeling, the accuracy of results from LC-UV is dramatically improved due to enhanced chromatographic resolution and validation by ESI-MS. For the first time ever, LC-UV may be used to accurately quantify 2-hydroxymethylglycerol, a branched chain alditol, D,L-glyceraldehyde, D,L-galactose, which represents the group of D,L-iditol/galactose/glucose/mannose and D,L-erythrose in formose reaction products.
本研究提出了一种液相色谱 - 紫外（LC - UV）方法，用于准确分析甲醛聚糖反应产物的总和参数。克服了气相色谱中遇到的局限性（例如分析物的热稳定性）。首先，糖醇进行O - 苯甲酰化并进行反相色谱分析。然后通过紫外检测，并通过电喷雾电离质谱（ESI - MS）进行验证。与标准的2,4 - 二硝基苯肼标记法相比，由于色谱分辨率的提高和ESI - MS验证，LC - UV结果的准确性得到了显著提高。首次可以使用LC - UV准确定量2 - 羟甲基甘油（一种支链糖醇）、D，L - 甘油醛、D，L - 半乳糖，它们代表了甲醛聚糖反应产物中的D，L - 艾杜糖醇/半乳糖/葡萄糖/甘露糖和D，L - 赤藓糖组。
