Science and Engineering Faculty, School of Earth, Environment and Biological Sciences, Queensland University of Technology, Brisbane, Qld, Australia.
Appl Environ Microbiol. 2013 Apr;79(7):2424-7. doi: 10.1128/AEM.03168-12. Epub 2013 Feb 1.
Environmental Burkholderia pseudomallei isolated from sandy soil at Castle Hill, Townsville, in the dry tropic region of Queensland, Australia, was inoculated into sterile-soil laboratory microcosms subjected to variable soil moisture. Survival and sublethal injury of the B. pseudomallei strain were monitored by recovery using culture-based methods. Soil extraction buffer yielded higher recoveries as an extraction agent than sterile distilled water. B. pseudomallei was not recoverable when inoculated into desiccated soil but remained recoverable from moist soil subjected to 91 days' desiccation and showed a growth response to increased soil moisture over at least 113 days. Results indicate that endemic dry tropic soil may act as a reservoir during the dry season, with an increase in cell number and potential for mobilization from soil into water in the wet season.
从澳大利亚昆士兰州干旱热带地区汤斯维尔市卡斯尔山的沙质土壤中分离出的环境型伯克霍尔德氏菌（Burkholderia pseudomallei）被接种到无菌土壤实验室微宇宙中，这些微宇宙的土壤湿度可变。通过基于培养的方法来监测 B. pseudomallei 菌株的存活和亚致死损伤的恢复情况。与无菌蒸馏水相比，土壤提取缓冲液作为提取剂可获得更高的回收率。当接种到干燥土壤中时，B. pseudomallei 不可恢复，但仍可从经过 91 天干燥的湿润土壤中恢复，并且在至少 113 天内对土壤湿度增加表现出生长反应。结果表明，地方性干旱热带土壤在旱季可能作为一个储存库，在雨季细胞数量增加，并有可能从土壤中转移到水中。
