土壤微宇宙中伯克霍尔德菌的形态变化与存活情况
Morphological Alteration and Survival of Burkholderia pseudomallei in Soil Microcosms.
作者信息
Kamjumphol Watcharaporn, Chareonsudjai Pisit, Taweechaisupapong Suwimol, Chareonsudjai Sorujsiri
机构信息
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand; Biofilm Research Group, Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand; Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand.
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand; Biofilm Research Group, Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand; Melioidosis Research Center, Khon Kaen University, Khon Kaen, Thailand
出版信息
Am J Trop Med Hyg. 2015 Nov;93(5):1058-65. doi: 10.4269/ajtmh.15-0177. Epub 2015 Aug 31.
The resilience of Burkholderia pseudomallei, the causative agent of melioidosis, was evaluated in control soil microcosms and in soil microcosms containing NaCl or FeSO4 at 30°C. Iron (Fe(II)) promoted the growth of B. pseudomallei during the 30-day observation, contrary to the presence of 1.5% and 3% NaCl. Scanning electron micrographs of B. pseudomallei in soil revealed their morphological alteration from rod to coccoid and the formation of microcolonies. The smallest B. pseudomallei cells were found in soil with 100 μM FeSO4 compared with in the control soil or soil with 0.6% NaCl (P < 0.05). The colony count on Ashdown's agar and bacterial viability assay using the LIVE/DEAD(®) BacLight(™) stain combined with flow cytometry showed that B. pseudomallei remained culturable and viable in the control soil microcosms for at least 120 days. In contrast, soil with 1.5% NaCl affected their culturability at day 90 and their viability at day 120. Our results suggested that a low salinity and iron may influence the survival of B. pseudomallei and its ability to change from a rod-like to coccoid form. The morphological changes of B. pseudomallei cells may be advantageous for their persistence in the environment and may increase the risk of their transmission to humans.
在30°C条件下，在对照土壤微观世界以及含有氯化钠或硫酸亚铁的土壤微观世界中，对类鼻疽病原体——伯克霍尔德菌的复原力进行了评估。在为期30天的观察期内，铁（亚铁）促进了伯克霍尔德菌的生长，这与1.5%和3%氯化钠的情况相反。土壤中伯克霍尔德菌的扫描电子显微镜图像显示，它们的形态从杆状变为球状，并形成了微菌落。与对照土壤或含有0.6%氯化钠的土壤相比，在含有100μM硫酸亚铁的土壤中发现的伯克霍尔德菌细胞最小（P<0.05）。使用Ashdown琼脂进行的菌落计数以及结合流式细胞术使用LIVE/DEAD（®）BacLight（™）染色进行的细菌活力测定表明，伯克霍尔德菌在对照土壤微观世界中至少120天仍可培养且存活。相比之下，含有1.5%氯化钠的土壤在第90天影响了它们的可培养性，在第120天影响了它们的活力。我们的结果表明，低盐度和铁可能会影响伯克霍尔德菌的存活及其从杆状转变为球状的能力。伯克霍尔德菌细胞的形态变化可能有利于它们在环境中的持久性，并可能增加它们传播给人类的风险。
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