刀豆凝集素对致敏和非致敏大鼠急性炎症的影响。
Effects of Canavalia lectins on acute inflammation in sensitized and non-sensitized rats.
机构信息
Institute of Biomedical Sciences, State University of Ceará, Av. Paranjana, 1700, 60740-000 Fortaleza, CE, Brazil.
出版信息
Inflammation. 2013 Jun;36(3):713-22. doi: 10.1007/s10753-013-9596-0.
The anti-inflammatory activity of Canavalia seed lectins (Canavalia gladiata [CGL], Canavalia maritima [ConM] and Canavalia brasiliensis [ConBr]) was evaluated by intravenous administration in rats. In non-sensitized rats, cellular edema elicited by carrageenan was reduced (45-51 %) by ConM and (44-59 %) by CGL. Osmotic edema elicited by dextran was reduced by ConM and CGL in 27 % and 29 %. ConM and CGL reduced the edema elicited by L-arginine in 53 % and that of prostaglandin E2 in 48 % and 36 %. Leukocyte migration elicited by carrageenan was reduced in 49 % by ConM and in 55 % by CGL (attenuated in 4× by glucose) and peritoneal TNF-α content in 82 %. In rats sensitized, ConM inhibited the paw edema and leukocyte migration elicited by ovalbumin in 34 % and 70 %. ConM and CGL are anti-inflammatory, mainly in cellular events mediated by prostaglandin E₂, nitric oxide and TNF-α in non-sensitized rats. However, only ConM is anti-inflammatory in sensitized rats. CGL effect involves the lectin domain.
采用静脉注射的方式在大鼠体内评估了刀豆球蛋白（Canavalia gladiata [CGL]、Canavalia maritima [ConM] 和 Canavalia brasiliensis [ConBr]）的抗炎活性。在非致敏大鼠中，ConM 降低了角叉菜胶诱导的细胞水肿（45-51%），CGL 降低了 44-59%。ConM 和 CGL 降低了右旋糖酐诱导的水肿，分别为 27%和 29%。ConM 和 CGL 降低了 L-精氨酸诱导的水肿（53%）和前列腺素 E2 诱导的水肿（48%和 36%）。ConM 降低了由角叉菜胶诱导的白细胞迁移（49%），CGL 降低了 55%（葡萄糖使其减弱 4 倍），ConM 和 CGL 还降低了 TNF-α在腹腔中的含量（82%）。在致敏大鼠中，ConM 抑制了卵清蛋白诱导的爪水肿和白细胞迁移，抑制率分别为 34%和 70%。ConM 和 CGL 在非致敏大鼠中主要通过前列腺素 E₂、一氧化氮和 TNF-α介导的细胞事件发挥抗炎作用。然而，只有 ConM 在致敏大鼠中具有抗炎作用。CGL 的作用涉及凝集素结构域。
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