使用特异性抗体同时检测大鼠黑质和中缝核中的色胺和多巴胺。

Simultaneous detection of tryptamine and dopamine in rat substantia nigra and raphe nuclei using specific antibodies.

作者信息

Dabadie H, Mons N, Geffard M

机构信息

Laboratoire d'Immunologie, Université de Bordeaux II, France.

出版信息

Brain Res. 1990 Mar 26;512(1):138-42. doi: 10.1016/0006-8993(90)91182-g.

DOI:10.1016/0006-8993(90)91182-g

PMID:2337800

Abstract

Using a double-labelling procedure, morphological relationships existing between dopaminergic and indoleaminergic neuronal systems in the rat brain were investigated. First, thanks to a tryptamine (T) antiserum, we visualized this indoleamine in all mesencephalic regions and showed that the T-immunoreactivity (IR) seems to overlap with the stainings observed from serotonin and 5-methoxytryptamine antisera. Secondly, using a monoclonal anti-dopamine (DA) antibody and our anti-T antibodies, the simultaneous and specific detection of these compounds enabled us to define the spatial relationships existing between the dopaminergic and tryptaminergic neuronal systems from the substantia nigra (SN) to the raphe nuclei. No co-localization existed, but the intensity of T-IR decreased from back to front, whereas the DA-staining decreased in the opposite way, indicating possible interactions at the end of the SN and the B9 area.

摘要

采用双标记法，对大鼠脑中多巴胺能和吲哚胺能神经元系统之间存在的形态学关系进行了研究。首先，借助色胺（T）抗血清，我们在所有中脑区域观察到了这种吲哚胺，并表明T免疫反应性（IR）似乎与血清素和5-甲氧基色胺抗血清所观察到的染色重叠。其次，使用单克隆抗多巴胺（DA）抗体和我们的抗T抗体，对这些化合物的同时特异性检测使我们能够确定从黑质（SN）到中缝核的多巴胺能和色胺能神经元系统之间存在的空间关系。不存在共定位，但T-IR的强度从前向后降低，而DA染色则以相反的方式降低，表明在SN末端和B9区域可能存在相互作用。