小鼠肝脏中碳水化合物代谢的调节。胰高血糖素对离体灌注肝脏中糖异生/糖酵解通量的影响。

Regulation of carbohydrate metabolism in mouse liver. Effect of glucagon on gluconeogenic/glycolytic flux in isolated perfused livers.

Cook D E, Schuster S M, Heidrick M L, Fienhold M A, Cook W, Markin R S

Department of Biochemistry, University of Nebraska Medical Center, Omaha 68105.

Comp Biochem Physiol B. 1979;64(1):33-9. doi: 10.1016/0305-0491(79)90180-9.

Abstract

Glucagon stimulated gluconeogenesis from both [U-14C]lactate and [14C]xylitol in isolated perfused mouse liver. 2. Addition of cyclic AMP also stimulated gluconeogenesis from [U-14C]lactate. 3. Glucagon caused a rapid (2.5 min) 12-fold increase in hepatic cyclic AMP but not cyclic GMP concentration. 4. Glucagon caused a rapid and stable decrease in hepatic fructose 1,6-diphosphatase activity measured in vitro. 5. The results are interpreted to indicate that glucagon stimulates hepatic gluconeogenesis in mice via cyclic AMP by two different mechanisms: (a) increased substrate uptake (i.e. utilization) and (b) increased gluconeogenic efficiency (i.e. inhibition of alternate substrate fates).

摘要

胰高血糖素刺激离体灌注小鼠肝脏中由[U-14C]乳酸盐和[14C]木糖醇生成葡萄糖异生作用。2. 添加环磷酸腺苷也刺激由[U-14C]乳酸盐生成葡萄糖异生作用。3. 胰高血糖素使肝脏中环磷酸腺苷浓度迅速（2.5分钟）增加12倍，但不影响环磷酸鸟苷浓度。4. 胰高血糖素使体外测定的肝脏果糖1,6-二磷酸酶活性迅速且稳定地降低。5. 结果被解释为表明胰高血糖素通过环磷酸腺苷经两种不同机制刺激小鼠肝脏中的葡萄糖异生作用：(a) 增加底物摄取（即利用）和(b) 提高糖异生效率（即抑制替代底物的命运）。