接受放疗的乳腺癌和妇科癌症患者淋巴细胞中三种功能预测性检测的比较分析
Comparative analysis of three functional predictive assays in lymphocytes of patients with breast and gynaecological cancer treated by radiotherapy.
作者信息
Padjas Anna, Kedzierawski Piotr, Florek Agnieszka, Kukolowicz Pawel, Kuszewski Tomasz, Góźdz Stanislaw, Lankoff Anna, Wojcik Andrzej, Lisowska Halina
机构信息
Department of Radiobiology and Immunology, Institute of Biology, Jan Kochanowski University, Kielce, Poland.
出版信息
J Contemp Brachytherapy. 2012 Dec;4(4):219-26. doi: 10.5114/jcb.2012.32556. Epub 2012 Dec 28.
PURPOSE
There is a need to develop predictive tests that would allow identifying cancer patients with a high risk of developing side effects to radiotherapy. We compared the predictive value of three functional assays: the G(0) aberration assay, the G(2) aberration assay and the alkaline comet assay in lymphocytes of breast cancer and gynaecological cancer patients.
MATERIAL AND METHODS
Peripheral blood was collected from 35 patients with breast cancer and 34 patients with gynaecological cancer before the onset of therapy. Chromosomal aberrations were scored in lymphocytes irradiated in the G(0) or G(2) phase of the cell cycle. DNA repair kinetics was performed with the alkaline comet assay following irradiation of unstimulated lymphocytes. The results were compared with the severity of early and late side effects to radiotherapy.
RESULTS
No correlation was observed between the results of the assays and the severity of side effects. Moreover, each assay identified different patients as radiosensitive.
CONCLUSIONS
There is no simple correlation between the in vitro sensitivity of lymphocytes and the risk of developing early and late side effects.
目的
需要开发预测性检测方法，以便能够识别出对放射治疗产生副作用风险较高的癌症患者。我们比较了三种功能检测方法的预测价值：G(0) 期染色体畸变检测、G(2) 期染色体畸变检测以及乳腺癌和妇科癌症患者淋巴细胞中的碱性彗星试验。
材料与方法
在治疗开始前，从35例乳腺癌患者和34例妇科癌症患者中采集外周血。对处于细胞周期G(0) 期或G(2) 期照射的淋巴细胞中的染色体畸变进行评分。在未刺激的淋巴细胞照射后，用碱性彗星试验进行DNA修复动力学检测。将结果与放射治疗早期和晚期副作用的严重程度进行比较。
结果
检测结果与副作用严重程度之间未观察到相关性。此外，每种检测方法所识别出的放射敏感患者各不相同。
结论
淋巴细胞的体外敏感性与发生早期和晚期副作用的风险之间不存在简单的相关性。
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