Oppitz U, Schulte S, Stopper H, Baier K, Müller M, Wulf J, Schakowski R, Flentje M
Department of Radiation Therapy, University of Würzburg, Josef-Schneider-Str. 11, 97080 Würzburg, Germany.
Int J Radiat Biol. 2002 Jul;78(7):611-6. doi: 10.1080/09553000210126466.
To compare colony-forming and comet assays on fibroblasts and lymphocytes of 32 breast cancer patients irradiated after breast-conserving operations and to correlate the results with acute clinical radiation reactions in the skin.
Skin fibroblasts were isolated and cultivated before radiotherapy and lymphocytes were drawn prior to the first and directly after the final external irradiation. The colony-forming assay was performed with fibroblasts and the comet assay with lymphocytes and fibroblasts of breast cancer patients according to standard protocols. The clinical radiation reactions of the patients were graded according to the RTOG system.
No significant correlation (p =0.09) was detected between clinical acute skin reactions and the in vitro clonogenic data in fibroblasts. Results of the comet assay in lymphocytes, however, showed a significant correlation (p <0.05) with the clinical data when patients were divided into two groups with average and elevated acute reactions. Apart from initial damage, fibroblasts did not show significant differences between the two patient groups. Repeated comet assays in lymphocytes of the same patient drawn before treatment and before and after external radiotherapy demonstrated good reproducibility of the test and no significant impact of preceding radiation treatment. There was a good correlation (r =0.65) between the comet assay results in fibroblasts and lymphocytes of the same individual.
In this cohort of patients, a significant correlation between the in vitro results of the comet assay in lymphocytes and clinical acute reactions was detected. The results of the comet assay and of fibroblast colony formation did not correlate with in vitro radiosensitivity.
比较32例保乳手术后接受放疗的乳腺癌患者的成纤维细胞和淋巴细胞的集落形成试验及彗星试验，并将结果与皮肤急性临床放射反应相关联。
放疗前分离并培养皮肤成纤维细胞，在首次和末次外照射前采集淋巴细胞。根据标准方案，对乳腺癌患者的成纤维细胞进行集落形成试验，对淋巴细胞和成纤维细胞进行彗星试验。根据RTOG系统对患者的临床放射反应进行分级。
未检测到临床急性皮肤反应与成纤维细胞体外克隆形成数据之间存在显著相关性（p = 0.09）。然而，当将患者分为急性反应平均和升高的两组时，淋巴细胞彗星试验结果与临床数据显示出显著相关性（p < 0.05）。除初始损伤外，两组患者的成纤维细胞未显示出显著差异。在同一患者治疗前、外照射前后采集的淋巴细胞中重复进行彗星试验，结果表明该试验具有良好的重复性，且先前的放射治疗对其无显著影响。同一患者的成纤维细胞和淋巴细胞的彗星试验结果之间存在良好的相关性（r = 0.65）。
在该队列患者中，检测到淋巴细胞彗星试验的体外结果与临床急性反应之间存在显著相关性。彗星试验结果和成纤维细胞集落形成结果与体外放射敏感性无关。
