在土耳其黑海中部地区首次使用实时逆转录聚合酶链反应对马匹进行西尼罗河病毒调查。
The first investigation of west nile virus in horses using real time rt-PCR in middle black sea region in Turkey.
作者信息
Yazici Zafer, Albayrak Harun, Ozan Emre, Gumusova Semra
机构信息
Department of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, Turkey.
出版信息
J Arthropod Borne Dis. 2012 Dec;6(2):151-5. Epub 2012 Dec 31.
BACKGROUND
West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals including humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study was to the investigate virological presence of WNV in horses in the Black Sea Region of Turkey using real time RT-PCR (rRT-PCR).
METHODS
Totally, 120 horse sera were collected equally from 4 provinces in Middle Black Sea Region of Turkey and investigated for WNV presence by Taqman based rRT-PCR.
RESULTS
WNV nucleic acid was not detected in any horse serum sample.
CONCLUSION
Although obtained result indicated no evidence of WNV-RNA in horses, Black Sea Region of Turkey is one of the suitable places for the WNV infection. For this reason, our research will continue for the determination of the viruses in vectors and susceptible animals such as horses, dogs, etc.
背景
西尼罗河病毒（WNV）是一种由蚊子传播的疾病，可在包括人类、狗、马、鸟类和爬行动物在内的哺乳动物中引起致命感染。虽然西尼罗河病毒感染通常无症状，但尤其可在人类和马中引发神经紊乱。本研究的目的是使用实时逆转录聚合酶链反应（rRT-PCR）调查土耳其黑海地区马匹中WNV的病毒学存在情况。
方法
总共从土耳其中黑海地区的4个省份平等收集了120份马血清，并通过基于Taqman的rRT-PCR检测WNV的存在情况。
结果
在任何马血清样本中均未检测到WNV核酸。
结论
虽然所得结果表明马匹中没有WNV-RNA的证据，但土耳其黑海地区是WNV感染的适宜地点之一。因此，我们的研究将继续进行，以确定媒介以及马、狗等易感动物中的病毒。
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