Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2013;48(4):429-36. doi: 10.1080/10934529.2013.729802.
Fluorescence spectrophotometry has been proposed as a quick screening technique for the measurement of naphthenic acids (NAs). To evaluate the feasibility of this application, the fluorescence emission spectra of NAs extracted from three oil sands process water sources were compared with that of commercial NAs. The NAs resulting from the bitumen extraction process cannot be differentiated because of the similarity of the fluorescence spectra. Separation of the fluorescent species in NAs using high performance liquid chromatography with fluorescence detector proved unsuccessful. The acidic fraction of NAs is fluorescent but the basic fraction of NAs is not fluorescent, implying that aromatic acids in NAs give rise to the fluorescent signals. The concentrations of NAs in oil sands process water were measured by Fourier transform infrared spectroscopy (FTIR), fluorescence spectrophotometry and ultra high performance liquid chromatography-time of flight/mass spectrometry (UPLC-TOF/MS). Commercial Merichem and Kodak NAs are the best standards to use when measuring NAs concentration with FTIR and fluorescence spectrophotometry. In addition, the NAs concentrations measured by fluorescence spectrophotometry are about 30 times higher than those measured by FTIR and UPLC-TOF/MS. The findings in this study underscore the limitation of fluorescence spectrophotometry in the measurement of NAs.
荧光分光光度法已被提议作为一种快速筛选技术，用于测量环烷酸（NA）。为了评估这种应用的可行性，比较了从三个油砂加工水源中提取的 NA 的荧光发射光谱与商业 NA 的荧光发射光谱。由于荧光光谱的相似性，无法区分来自沥青萃取过程的 NA。使用带有荧光检测器的高效液相色谱法分离 NA 中的荧光物质证明是不成功的。NA 的酸性部分具有荧光性，但碱性部分没有荧光性，这意味着 NA 中的芳香酸产生了荧光信号。通过傅里叶变换红外光谱（FTIR）、荧光分光光度法和超高效液相色谱-飞行时间/质谱法（UPLC-TOF/MS）测量油砂加工水中的 NA 浓度。当使用 FTIR 和荧光分光光度法测量 NA 浓度时，商业 Merichem 和 Kodak NA 是最佳的标准。此外，荧光分光光度法测量的 NA 浓度比 FTIR 和 UPLC-TOF/MS 测量的浓度高约 30 倍。本研究的结果强调了荧光分光光度法在测量 NA 方面的局限性。
