Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Apr;106:190-6. doi: 10.1016/j.saa.2012.12.076. Epub 2013 Jan 4.
The present study focuses on the rapid synthesis of gold nanoparticles (AuNP) using the aqueous extract of Cissus quadrangularis (CQE) by microwave irradiation. The UV-Visible spectroscopy of the solution obtained from reduction of hydrogen tetrachloroaurate (HAuCl4) by CQE revealed a sharp surface plasmon resonance (SPR) peak at 530 nm confirming the presence of AuNP. The formation of AuNP was optimal at a pH of 9. The AuNP was characterised by FT-IR, SEM, HR-TEM, SAED, XRD, TGA, DLS and Zeta potential measurements. The results indicated that microwave assisted synthesis produced well dispersed, small sized, uniform nanoparticles when compared to conventional room temperature synthesis. The spherical nanoparticle had an average size of 12.0±3.2 nm as revealed through TEM. The crystalline nature of AuNP was confirmed through HR-TEM, SAED and XRD. The FT-IR and TGA data revealed the presence of the CQE components on the surface of the AuNP particles which serve as the capping agent. Upon incubation, the particles did not lyse the red blood corpuscles (RBCs) indicating that they are biocompatible. A possible mechanism for the formation of AuNP in the presence of CQE is proposed.
本研究采用微波辐射法，利用四方葡萄（CQE）的水提物快速合成金纳米粒子（AuNP）。通过 CQE 将四氯金酸（HAuCl4）还原得到的溶液的紫外-可见光谱显示，在 530nm 处有一个尖锐的表面等离子体共振（SPR）峰，证实存在 AuNP。AuNP 的最佳形成条件为 pH 值 9。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、高分辨率透射电子显微镜（HR-TEM）、选区电子衍射（SAED）、X 射线衍射（XRD）、热重分析（TGA）、动态光散射（DLS）和 Zeta 电位测量对 AuNP 进行了表征。结果表明，与传统的室温合成相比，微波辅助合成产生了分散性更好、尺寸更小、更均匀的纳米粒子。通过 TEM 揭示，球形纳米粒子的平均粒径为 12.0±3.2nm。通过 HR-TEM、SAED 和 XRD 证实了 AuNP 的结晶性质。FT-IR 和 TGA 数据表明，CQE 成分存在于 AuNP 颗粒表面，起到了包覆剂的作用。孵育后，颗粒没有裂解红细胞（RBCs），表明它们具有生物相容性。提出了在 CQE 存在下形成 AuNP 的可能机制。
