Lachno D R, Patel N, Rose M L, Yacoub M H
Thoracic and Cardiac Surgical Unit, Harefield Hospital, Middlesex, U.K.
J Chromatogr. 1990 Jan 26;525(1):123-32. doi: 10.1016/s0378-4347(00)83385-x.
Transplant patients receiving the immunosuppressive drug cyclosporin A require regular monitoring to maintain levels within a narrow therapeutic range. A stable, accurate and reproducible high-performance liquid chromatographic method for analysis of cyclosporin A in whole blood has been developed using the Varian Advanced Automated Sample Processor. Starting with 200 microliters of blood, absolute recovery of both cyclosporin A and the internal standard was 81% with a detection limit of 12.5 ng/ml. The assay is perfectly linear over the range 0-1000 ng/ml (r2 = 1.0). At a concentration of 250 ng/ml, the coefficient of variation, both between samples and between assays, is 1.87%. Chromatographic cycle time is 10.2 min per sample. Up to eighty samples can be processed by one person in a working day, with final results within 16 h.
接受免疫抑制药物环孢素A治疗的移植患者需要定期监测，以将血药浓度维持在较窄的治疗范围内。使用瓦里安高级自动进样器开发了一种稳定、准确且可重复的高效液相色谱法，用于分析全血中的环孢素A。以200微升血液为起始样本，环孢素A和内标物的绝对回收率均为81%，检测限为12.5纳克/毫升。该测定法在0 - 1000纳克/毫升范围内呈完美线性（r2 = 1.0）。在浓度为250纳克/毫升时，样本间和测定间的变异系数为1.87%。每个样本的色谱分析周期时间为10.2分钟。一个人在一个工作日内可处理多达80个样本，16小时内可得出最终结果。
