Shinohara Y, Kirii N, Tamaoki H, Magara H, Baba S
Tokyo College of Pharmacy, Japan.
J Chromatogr. 1990 Jan 26;525(1):93-104. doi: 10.1016/s0378-4347(00)83382-4.
A method for the stereoselective assay of the (+)- and (-)-enantiomers of suprofen and [2H3]suprofen in human plasma was developed using gas chromatography-mass spectrometry-selected-ion monitoring. (+/-)-[2H7]Suprofen was used as an internal standard. The method involved diethyl ether extraction and chiral derivatization with S-(-)-1-(naphthyl)ethylamine to form diastereomeric amide. The diastereoisomers were separated on a capillary gas chromatograph-mass spectrometer. Quantitation was achieved by selected-ion monitoring of the quasi-molecular ions of the diastereoisomers. The sensitivity, specificity, accuracy and reproducibility of the method were demonstrated to be satisfactory for application to pharmacokinetic studies of suprofen enantiomers.
建立了一种采用气相色谱 - 质谱 - 选择离子监测法对人血浆中舒洛芬及其[2H3]舒洛芬的（+）-和（-）-对映体进行立体选择性测定的方法。（±）-[2H7]舒洛芬用作内标。该方法包括用乙醚萃取，并用S - （-）-1 - （萘基）乙胺进行手性衍生化以形成非对映体酰胺。非对映异构体在毛细管气相色谱 - 质谱仪上分离。通过对非对映异构体的准分子离子进行选择离子监测来实现定量。该方法的灵敏度、特异性、准确性和重现性经证明适用于舒洛芬对映体的药代动力学研究，令人满意。
