用 14C 标记的乳糖研究固定在低成本载体上的发酵乳杆菌的乳清发酵。
Study of whey fermentation by kefir immobilized on low cost supports using 14C-labelled lactose.
机构信息
Department of Chemistry, University of Patras, Patras, Greece.
出版信息
Bioresour Technol. 2013 Oct;145:326-30. doi: 10.1016/j.biortech.2012.12.131. Epub 2013 Jan 7.
Brewer's Spent Grains (BSG) and Malt Spent Rootlets (MSR) were used as supports for kefir cells immobilization and the role of lactose uptake rate by kefir in the positive activity of produced biocatalysts during whey fermentation was investigated. Lactose uptake rate by the immobilized cells was recorded using (14)C-labelled lactose and the effect of various conditions (pH, temperature and kind of support) on it and consequently on fermentation time and ethanol production was examined. The results showed that lactose uptake rate was correlated to fermentation rate and increased as temperature was increased up to 30°C at pH 5.5. The same results have been recently noticed by using biocatalysts with Delignified Cellulosic Materials (DCM) and Gluten Pellets (GP), but fermentation time of about 7h by kefir immobilized on DCM and BSG resulted to two fold lower than that on GP and MSR. The highest alcohol concentration was observed by MSR.
啤酒糟（BSG）和麦芽废根（MSR）被用作发酵乳球菌细胞固定化的载体，研究了发酵乳球菌细胞在乳清发酵过程中乳糖摄取率对生物催化剂活性的影响。使用（14）C 标记的乳糖记录固定化细胞的乳糖摄取率，并研究了各种条件（pH 值、温度和载体类型）对其的影响，以及对发酵时间和乙醇产量的影响。结果表明，乳糖摄取率与发酵速率相关，在 pH 值为 5.5 时，温度升高至 30°C 时乳糖摄取率增加。使用经过脱木质素的纤维素材料（DCM）和面筋颗粒（GP）的生物催化剂也得到了同样的结果，但固定化发酵乳球菌在 DCM 和 BSG 上的发酵时间比在 GP 和 MSR 上低两倍。MSR 观察到的酒精浓度最高。
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