Bomford R, Aston R
Wellcome Research Laboratories, Beckenham, Kent.
J Endocrinol. 1990 Apr;125(1):31-8. doi: 10.1677/joe.0.1250031.
The biological activity of growth hormones can be enhanced by complexing with monoclonal antibodies of appropriate specificity. In order to define the regions associated with the phenomenon, site-directed antisera to ovine GH (oGH) were prepared by vaccination of sheep with synthetic peptides. Peptides from six distinct regions of the oGH molecule raised antibodies which recognized the hormone in solid-phase radioimmunoassay; however, only one peptide elicited high-affinity antibody as determined by liquid-phase assay. This peptide, corresponding to amino acid sequence 35-53, resulted in circulating hormone antibody in the majority of vaccinated sheep. Immunoglobulin prepared from the serum of immunized animals produced an enhancement of the somatotrophic activity of exogenously administered GH in dwarf mice as determined by the incorporation of [35S]sulphate into costal cartilage. The identification of an antigenic peptide sequence from oGH/bovine GH which elicits enhancing antisera, raises the possibility of a growth-promotion vaccine.
生长激素的生物活性可通过与具有适当特异性的单克隆抗体复合来增强。为了确定与该现象相关的区域，通过用合成肽免疫绵羊制备了针对绵羊生长激素（oGH）的定点抗血清。来自oGH分子六个不同区域的肽引发了在固相放射免疫测定中识别该激素的抗体；然而，通过液相测定确定只有一种肽引发了高亲和力抗体。该肽对应于氨基酸序列35 - 53，在大多数接种疫苗的绵羊中产生了循环激素抗体。从免疫动物血清中制备的免疫球蛋白增强了外源性给予侏儒小鼠的GH的促生长活性，这是通过将[35S]硫酸盐掺入肋软骨来确定的。从oGH/牛生长激素中鉴定出一种能引发增强抗血清的抗原肽序列，增加了生长促进疫苗的可能性。
