Nehmé Reine, Perrin Catherine
Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans, UMR CNRS 7311, Orléans, France.
Methods Mol Biol. 2013;984:191-206. doi: 10.1007/978-1-62703-296-4_14.
Capillary electrophoresis (CE) is an interesting technique for protein and peptide analysis. However, one of the major problems concerns sample adsorption on the internal capillary wall. The use of non-covalent coatings using highly charged polyelectrolytes is an efficient, simple, and fast approach to reduce peptide and protein adsorption phenomena. We have studied in a systematic manner the effect of coating conditions on the stability and efficiency of multilayer coatings using poly(diallyldimethylammonium) chloride (PDADMAC) as polycation and polystyrene sulfonate (PSS) as polyanion. When optimal conditions defined in the protocols are used, very stable coatings are obtained and adsorption phenomena are eliminated. The coatings are stable over a large range of pH buffer (2-10) and in the presence of organic solvent. Hundreds of analyses can be performed without coating regeneration. Coated capillaries can be easily stored and reused.
毛细管电泳（CE）是一种用于蛋白质和肽分析的有趣技术。然而，主要问题之一是样品在毛细管内壁上的吸附。使用带高电荷聚电解质的非共价涂层是减少肽和蛋白质吸附现象的一种有效、简单且快速的方法。我们系统地研究了涂层条件对以聚二烯丙基二甲基氯化铵（PDADMAC）为聚阳离子、聚苯乙烯磺酸盐（PSS）为聚阴离子的多层涂层稳定性和效率的影响。当使用方案中定义的最佳条件时，可获得非常稳定的涂层并消除吸附现象。这些涂层在很大的pH缓冲范围（2 - 10）以及有机溶剂存在的情况下都很稳定。无需涂层再生即可进行数百次分析。涂覆的毛细管易于储存和重复使用。
