Department of Molecular Biology and Genetics, Aarhus University, Denmark.
Prenat Diagn. 2013 Mar;33(3):284-91. doi: 10.1002/pd.4060. Epub 2013 Feb 7.
Cells of foetal origin circulating in the maternal peripheral bloodstream present a unique source for non-invasive prenatal diagnostics. The aims of this study were to raise antibodies against identified circulating foetal cells from the maternal blood, test the properties of these antibodies and to determine the foetal cell type recognised by the antibodies.
Cells from a male foetus were identified in a maternal blood sample by FISH analysis of the X- and Y- chromosomes. The identified cells were subjected to phage display selection using a novel single cell selection strategy. Selected antibodies were tested by immunocytochemistry on foetal and adult tissue arrays, an endothelial cell line, and peripheral blood mononuclear cells.
Three identified foetal cells subjected to antibody selection, yielded a total of 12 antibodies. Three antibodies gave distinct staining patterns on tissue arrays, and endothelial cells. One antibody, SF1.3, shows specific staining of a subpopulation of peripheral blood mononuclear cells, including a fraction of CD34 positive cells.
These findings indicate that the identified foetal cells could have been progenitor cells of haematopoietic origin. The antibody SF1.3 could be a potential tool toward non-invasive prenatal diagnostics.
循环于母体外周血液中的胎儿来源细胞为非侵入性产前诊断提供了独特的来源。本研究的目的是针对从母体血液中分离出的已鉴定的循环胎儿细胞产生抗体，测试这些抗体的特性，并确定抗体所识别的胎儿细胞类型。
通过 X 和 Y 染色体的 FISH 分析，从母体血液样本中鉴定出男性胎儿的细胞。利用新型单细胞选择策略对鉴定出的细胞进行噬菌体展示选择。通过免疫细胞化学染色法对胎儿和成人组织芯片、内皮细胞系和外周血单核细胞进行所选抗体的测试。
对 3 个经抗体选择的鉴定胎儿细胞，共产生了 12 种抗体。3 种抗体在组织芯片和内皮细胞上呈现出独特的染色模式。一种抗体 SF1.3 特异性地染色外周血单核细胞的一个亚群，包括一部分 CD34 阳性细胞。
这些发现表明，鉴定出的胎儿细胞可能是造血祖细胞。抗体 SF1.3 可能是一种非侵入性产前诊断的潜在工具。
