Podgórska K, Kamieniecka K, Stadejek T, Pejsak Z
National Veterinary Research Institute, Department of Swine Diseases, Partyzantow 57, 24-100 Pulawy, Poland.
Pol J Vet Sci. 2012;15(4):615-20. doi: 10.2478/v10181-012-0097-0.
Classical swine fever (CSF) is a notifiable, highly contagious disease of swine controlled mainly with costly administrative methods. Swine may be infected not only with classical swine fever virus (CSFV), but also with other, non porcine, genetically and antigenically related pestiviruses. Differentiation of infections with CSFV and other pestiviruses is a crucial element of diagnostics. In the present study two real-time PCR methods and conventional one-tube nested PCR for specific detection of CSFV were compared. Additionally, two methods designed for detection of all pestivirus species real-time SYBR Green I and one-tube nested PCR were included into the study. Analyzed methods varied considerably regarding their sensitivity and specificity, what suggests that careful selection of diagnostic methods and their evaluation on a regular basis is necessary.
经典猪瘟（CSF）是一种须上报的、高度传染性的猪病，主要通过成本高昂的行政管理方法进行控制。猪不仅可能感染经典猪瘟病毒（CSFV），还可能感染其他与猪无关、在基因和抗原上相关的瘟病毒。区分CSFV感染与其他瘟病毒感染是诊断的关键要素。在本研究中，对两种用于特异性检测CSFV的实时PCR方法和传统单管巢式PCR进行了比较。此外，还将两种设计用于检测所有瘟病毒种类的方法——实时SYBR Green I和单管巢式PCR纳入了研究。所分析的方法在灵敏度和特异性方面差异很大，这表明有必要仔细选择诊断方法并定期对其进行评估。
