大肠杆菌γ-谷氨酰转肽酶的热变性与复性
Thermal denaturation and renaturation of γ-glutamyltranspeptidase of Escherichia coli.
作者信息
Ho Thao Van, Kamei Kaeko, Wada Kei, Fukuyama Keiichi, Suzuki Hideyuki
机构信息
Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan.
出版信息
Biosci Biotechnol Biochem. 2013;77(2):409-12. doi: 10.1271/bbb.120780. Epub 2013 Feb 7.
Heat-treated γ-glutamyltranspeptidase of Escherichia coli recovered enzymatic activity after incubation at 4 °C, while heat-treated γ-glutamyltranspeptidase of Bacillus subtilis did not. Fluorescent spectra, CD spectra, and native polyacrylamide gel electrophoresis analysis suggested that the dimer of E. coli γ-glutamyltranspeptidase was separated into protomers by heat-treatment, but was renatured by incubation at 4 °C.
大肠杆菌经热处理的γ-谷氨酰转肽酶在4℃孵育后恢复了酶活性，而枯草芽孢杆菌经热处理的γ-谷氨酰转肽酶则没有。荧光光谱、圆二色光谱和天然聚丙烯酰胺凝胶电泳分析表明，大肠杆菌γ-谷氨酰转肽酶的二聚体经热处理后分离成单体，但在4℃孵育后复性。
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