Homo-Delarche F, Gagnerault M C, Bach J F, Dardenne M
INSERM U 25, Hôpital Necker, Paris, France.
Prostaglandins. 1990 Mar;39(3):299-318. doi: 10.1016/0090-6980(90)90049-2.
Prostaglandins (PGs) have been assumed to play a role in the biological activity of thymic hormones (TH). Indeed, it has been shown that type E-PGs are able to mimic the action of several TH. Moreover, indomethacin interferes in the rosette assay, which still represents the most commonly used bioassay for the evaluation of TH and, in particular thymulin levels, in biological fluids. Previously, our attempt to modulate PG production by different TH showed that none of the TH tested affect PGE2, 6-keto-PGF1 alpha, PGF2 alpha and TXB2 production by spleen cells from control and thymectomized (Tx) mice, while indomethacin was able to inhibit the spontaneous PG production. Here, we investigated a possible role for each endogenously produced PG in the experimental conditions of the rosette assay, in order to define: 1) whether or not there was a specificity of action of a given PG; and 2) to analyze the pattern of action between thymulin and the endogenously produced PGs. We demonstrated that PGE2 and 6-keto-PGF1 alpha are the PGs which are physiologically involved in the rosette assay, according to their levels of endogenous production, and that they are able to synergize with thymulin. This synergy was demonstrated in two ways: 1) by adding anti-PGE2 and anti-6-keto PGF1 alpha-antibodies, which prevent part of the thymulin effect, or 2) by simultaneous addition of PG and thymulin, at concentrations far lower than those which correspond to their thymulin-like effect. Moreover, PGE2 addition, at concentration close to that found to be endogenously produced, partially reversed the indomethacin-induced effect in the rosette assay. In conclusion, if PGs do not act as mediators of thymulin, they are able to synergize in one of its biological action.
前列腺素（PGs）被认为在胸腺激素（TH）的生物活性中起作用。事实上，已经表明E型前列腺素能够模拟几种胸腺激素的作用。此外，吲哚美辛会干扰玫瑰花结试验，该试验仍是评估生物体液中胸腺激素，特别是胸腺素水平最常用的生物测定法。此前，我们试图通过不同的胸腺激素调节前列腺素的产生，结果表明，所测试的任何一种胸腺激素均不影响对照小鼠和胸腺切除（Tx）小鼠脾脏细胞产生前列腺素E2、6-酮-前列腺素F1α、前列腺素F2α和血栓素B2，而吲哚美辛能够抑制前列腺素的自发产生。在此，我们研究了每种内源性产生的前列腺素在玫瑰花结试验的实验条件下可能发挥的作用，以便确定：1）特定前列腺素的作用是否具有特异性；2）分析胸腺素与内源性产生的前列腺素之间的作用模式。我们证明，根据内源性产生水平，前列腺素E2和6-酮-前列腺素F1α是生理上参与玫瑰花结试验的前列腺素，并且它们能够与胸腺素协同作用。这种协同作用通过两种方式得到证明：1）添加抗前列腺素E2和抗6-酮-前列腺素F1α抗体，这会部分阻止胸腺素的作用；2）同时添加前列腺素和胸腺素，其浓度远低于对应其胸腺素样作用的浓度。此外，添加浓度接近内源性产生浓度的前列腺素E2，可部分逆转吲哚美辛在玫瑰花结试验中诱导的效应。总之，如果前列腺素不是作为胸腺素的介质发挥作用，它们能够在其一种生物学作用中协同发挥作用。
