Roldán-Pijuán M, Alcudia-León Mc, Lucena R, Cárdenas S, Valcárcel M
Department of Analytical Chemistry, Institute of Fine Chemistry & Nanochemistry, Marie Curie Building, Campus de Rabanales, University of Córdoba, 14071, Córdoba, Spain.
Bioanalysis. 2013 Feb;5(3):307-15. doi: 10.4155/bio.12.312.
In this article, stir-membrane liquid microextraction is adapted for the analysis of volume-limited biological samples, using as a model analytical problem the determination of paracetamol in human saliva by LC with UV. A three-phase microextraction mode is used for the extraction of the target analyte, taking advantage of its acid-base properties.
All the variables involved in the extraction have been studied and optimized in depth. The method has been analytically characterized on the basis of its linearity, sensitivity and precision. The LOD is 0.5 µg/l while the repeatability, expressed as RSD, is better than 14.2 %. A tenfold preconcentration factor was obtained, which involves an absolute recovery value of 25%. Moreover, the relative recovery is very close to the 100%.
Finally, the proposed method has been used to perform a PK study of paracetamol.
在本文中，搅拌膜液液微萃取法适用于分析体积有限的生物样品，以液相色谱 - 紫外检测法测定人唾液中对乙酰氨基酚作为模型分析问题。利用目标分析物的酸碱性质，采用三相微萃取模式萃取目标分析物。
对萃取过程中涉及的所有变量进行了深入研究和优化。该方法基于线性、灵敏度和精密度进行了分析表征。检测限为0.5μg / l，以相对标准偏差（RSD）表示的重复性优于14.2％。获得了10倍的预富集因子，绝对回收率为25％。此外，相对回收率非常接近100％。
最后，所提出的方法已用于对乙酰氨基酚的药代动力学研究。
