Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Warsaw, Poland.
Exp Gerontol. 2013 Apr;48(4):401-7. doi: 10.1016/j.exger.2013.01.015. Epub 2013 Feb 5.
The physiological mechanisms that promote longevity remain unclear. It has been suggested that insulin sensitivity is preserved in centenarians, whereas typical aging is accompanied by increasing insulin resistance. The oldest-old individuals display raised total adiponectin levels, despite the potential correlation between enhanced adiponectin and all-cause and cardiovascular mortality.
To evaluate the level of adiponectin and its isoforms in sera of centenarians and to assess associations between adiponectin and metabolic parameters.
A group of 58 Polish centenarians (50 women and 8 men, mean age 101±1.34 years) and 68 elderly persons (55 women and 13 men, mean age 70±5.69 years) as controls.
Serum samples were analyzed to evaluate the following parameters: adiponectin array (total adiponectin, HWM-, MMW- and LMW-adiponectin; all by ELISA methods), insulin (by IRMA methods), glucose and lipid profiles. HOMA-IR was calculated. Clinical data were collected. Statistical analyses were performed.
The concentrations of all adiponectin isoforms were significantly higher in the oldest-old participants. In the centenarian group, total adiponectin positively correlated with age and HDL-cholesterol, and HMW-adiponectin was negatively associated with insulin and triglycerides. The long-lived participants had a lower incidence of hypertension, type 2 diabetes, overweight and obesity, with lower concentrations of serum glucose and insulin, and reduced HOMA-IR.
Our findings support the thesis that centenarians possess a different adiponectin isoform pattern and have a favorable metabolic phenotype in comparison with elderly individuals. However, additional work is necessary to understand the relevance of these findings to longevity.
促进长寿的生理机制尚不清楚。有人认为，百岁老人的胰岛素敏感性得到了保留，而典型的衰老伴随着胰岛素抵抗的增加。尽管增强的脂联素与全因和心血管死亡率之间存在潜在关联，但最年长的个体显示出升高的总脂联素水平。
评估百岁老人血清中脂联素及其异构体的水平，并评估脂联素与代谢参数之间的相关性。
一组 58 名波兰百岁老人（50 名女性和 8 名男性，平均年龄 101±1.34 岁）和 68 名老年人（55 名女性和 13 名男性，平均年龄 70±5.69 岁）作为对照组。
分析血清样本以评估以下参数：脂联素数组（总脂联素、高 MW-、中 MW-和低 MW-脂联素；均通过 ELISA 方法）、胰岛素（通过 IRMA 方法）、血糖和血脂谱。计算 HOMA-IR。收集临床数据。进行统计分析。
所有脂联素异构体的浓度在最年长的参与者中均显著升高。在百岁老人组中，总脂联素与年龄和 HDL 胆固醇呈正相关，高 MW 脂联素与胰岛素和甘油三酯呈负相关。长寿参与者高血压、2 型糖尿病、超重和肥胖的发生率较低，血清葡萄糖和胰岛素浓度较低，HOMA-IR 降低。
我们的研究结果支持这样的论点，即与老年人相比，百岁老人具有不同的脂联素异构体模式和有利的代谢表型。然而，需要进一步的研究来理解这些发现与长寿的相关性。
