Dholpuria S, Vyas S, Purohit G N, Pathak K M L
Veterinary Hospital Garabdesar, District Bikaner, Rajasthan, India.
J Ultrasound. 2012 Jun;15(2):135-41. doi: 10.1016/j.jus.2012.02.008. Epub 2012 Mar 9.
The present study examined the effect of melatonin implants on follicle growth in dromedary camels two months ahead of their natural breeding season (December to March). Female camels (n = 6) were treated with melatonin implants at the dose rate of 1 implant per 28 kg body weight sc. Control camels (n = 6) were administered an SC placebo implant of 8 ml vitamin A. Ovarian ultrasonography was performed at weekly interval upto 7 weeks. Camels were mated with virile stud when a follicle (≥10 mm) was visible on either of the ovaries. Blood was collected on day 7, 9, 15, 20, 25 and 30 for assay of plasma progesterone and sonography performed at the same time. Small follicles (2-3 mm) appeared around the periphery of ovaries in 83.3% of camels by day 7 and in 100% camels by day 14. By the end of 7th week an ovulatory size follicle (≥1.0 cm) could be observed in 83.3% of treated camels, and these camels were mated with virile studs. In control group, small follicles appeared at the periphery of ovaries only in 66.6% camels but did not progress in growth except in one camel (16.6%) however, ovulating size (≥10 mm) follicle was not observed in any camel by the end of 7th week. All treated camels ovulated and one treated camel became pregnant while early embryonic death occurred in one camel. Non-pregnant camels of both groups were mated during the breeding season. All camels of treatment group and 33.33% camels of control group became pregnant by the end of breeding season (April 2010). It was concluded that melatonin implants can augment the follicle growth in lactating camels ahead of the breeding season and pregnancy can occur on mating. Fertility of treated camels during the breeding season is improved.
本研究检测了褪黑素植入剂对单峰骆驼在其自然繁殖季节（12月至3月）前两个月卵泡生长的影响。雌性骆驼（n = 6）以每28千克体重皮下植入1个植入剂的剂量接受褪黑素植入剂治疗。对照骆驼（n = 6）接受8毫升维生素A的皮下安慰剂植入。每周进行一次卵巢超声检查，持续7周。当任一卵巢上可见卵泡（≥10毫米）时，骆驼与有生育能力的种公骆驼交配。在第7、9、15、20、25和30天采集血液用于检测血浆孕酮，并同时进行超声检查。到第7天，83.3%的骆驼卵巢周边出现小卵泡（2 - 3毫米），到第14天，100%的骆驼出现小卵泡。到第7周结束时，83.3%的治疗组骆驼可观察到排卵大小的卵泡（≥1.0厘米），这些骆驼与有生育能力的种公骆驼交配。在对照组中，仅66.6%的骆驼卵巢周边出现小卵泡，但除一只骆驼（16.6%）外，卵泡生长未进展，然而，到第7周结束时，未在任何骆驼中观察到排卵大小（≥10毫米）的卵泡。所有治疗组骆驼均排卵，一只治疗组骆驼怀孕，而一只骆驼发生早期胚胎死亡。两组未怀孕的骆驼在繁殖季节进行交配。到繁殖季节结束时（2010年4月），治疗组所有骆驼和对照组33.33%的骆驼怀孕。得出的结论是，褪黑素植入剂可在繁殖季节前促进泌乳骆驼的卵泡生长，交配后可实现怀孕。治疗组骆驼在繁殖季节的生育能力得到提高。
