伴刀豆球蛋白A抑制组织因子凝血活性。
Concanavalin A inhibits tissue factor coagulant activity.
作者信息
Pitlick F A
出版信息
J Clin Invest. 1975 Jan;55(1):175-9. doi: 10.1172/JCI107908.
Abstract
Concanavalin A (con A) is a potent inhibitor of coagulant activity of native tissue factor. Coagulant activity is recovered by addition of alpha-methyl-D-glucoside to inhibited tissue factor. Inclusion of alpha-methyl-D-glucose during incubation of con A with tissue factor preserves coagulant activity. These data suggest that con A interacts reversibly with a carbohydrate residue in such a way as to inhibit coagulant activity of the molecule. Purified tissue factor apoprotein has been recombined with mixed brain phospholipids or purified phospholipids (phosphatidyl ethanolamine or a mixture of phosphatidyl choline with phosphatidyl serine). These preparations were also completely but reversibly inhibited by con A. Thus, purified tissue factor apoprotein appears to donate the affected carbohydrate residue.
摘要
伴刀豆球蛋白A（伴刀豆凝集素A）是天然组织因子凝血活性的强效抑制剂。通过向受抑制的组织因子中添加α-甲基-D-葡萄糖苷可恢复凝血活性。在伴刀豆凝集素A与组织因子孵育期间加入α-甲基-D-葡萄糖可保留凝血活性。这些数据表明，伴刀豆凝集素A与碳水化合物残基可逆性相互作用，从而抑制该分子的凝血活性。纯化的组织因子载脂蛋白已与混合脑磷脂或纯化磷脂（磷脂酰乙醇胺或磷脂酰胆碱与磷脂酰丝氨酸的混合物）重新组合。这些制剂也被伴刀豆凝集素A完全但可逆地抑制。因此，纯化的组织因子载脂蛋白似乎提供了受影响的碳水化合物残基。
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Characteristics and lipid requirements of coagulant proteins extracted from lung and brain: the specifity of protein component of tissue factor.从肺和脑中提取的凝血蛋白的特性及脂质需求：组织因子蛋白质成分的特异性
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