一株链霉菌次级代谢产物 SEM-1-111 对 Wistar 大鼠全层皮肤切除创面的愈合潜力。
Healing potential of a Streptomyces sp. secondary metabolite, SEM-1-111, on experimental full-thickness excision cutaneous wounds in Wistar rats.
机构信息
Department of Molecular Cell Biology, North-west Branch, Razi Vaccine and Serum Research Institute, Marand, Iran.
出版信息
Clin Exp Dermatol. 2013 Mar;38(2):178-84. doi: 10.1111/ced.12026.
BACKGROUND
The potent biomedical properties of Streptomyces secondary metabolites make them a possible source of promising new targets to use as wound-healing-promoting agents.
AIM
To investigate the properties of a Streptomyces sp. secondary metabolite, the SEM-1-111 fraction, in the healing full-thickness cutaneous wounds.
METHODS
In this experiment, creams were prepared using a Eucerin ointment base with 5%, 10% or 15% SEM-1-111 added. Circular excision wounds were surgically created on the back of Wistar rats, and the creams were applied daily to the wound area. The negative control group was treated with the vehicle, and the positive control group with a commercial phenytoin 1% cream. We analysed planimetric and histological parameters, and the expression of procollagen-I mRNA.
RESULTS
SEM-1-111 substantially influenced wound healing. The cream containing the highest dose of metabolite (15%) significantly decreased wound size during the study, and histological evaluation showed beneficial effects of this concentration in the wound-healing process. Procollagen-I mRNA levels were significantly increased in both the 15% SEM-1-111 group and the phenytoin group compared with the vehicle control on postoperative day 7.
CONCLUSIONS
Regular topical application of the Streptomyces sp. secondary metabolite SEM-1-111 fraction at a concentration of 15% accelerates the wound-healing process.
背景
链霉菌次级代谢产物具有强大的生物医学特性，因此它们可能成为有前途的新靶点的来源，可作为促进伤口愈合的药物。
目的
研究链霉菌次级代谢产物 SEM-1-111 馏分在促进全层皮肤伤口愈合中的特性。
方法
在这项实验中，使用含有 5%、10%或 15% SEM-1-111 的优色林软膏基质制备乳膏。在 Wistar 大鼠背部通过手术制造圆形切除伤口，并将乳膏每天涂抹在伤口区域。阴性对照组用赋形剂处理，阳性对照组用商业苯妥英 1%乳膏处理。我们分析了平面和组织学参数以及前胶原-I mRNA 的表达。
结果
SEM-1-111 对伤口愈合有显著影响。含有最高剂量代谢物（15%）的乳膏在研究期间显著减小了伤口面积，组织学评估显示这种浓度对伤口愈合过程有有益的影响。与赋形剂对照组相比，术后第 7 天，15%SEM-1-111 组和苯妥英组的前胶原-I mRNA 水平均显著升高。
结论
以 15%浓度定期局部应用链霉菌次级代谢产物 SEM-1-111 馏分可加速伤口愈合过程。
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