嗅觉介质样3（OLFML3）基因在狒狒和人类眼部组织中的表达：角膜、晶状体、葡萄膜和视网膜。
Olfactomedin-like 3 (OLFML3) gene expression in baboon and human ocular tissues: cornea, lens, uvea, and retina.
作者信息
Rodríguez-Sánchez I P, Garza-Rodríguez M L, Mohamed-Noriega K, Voruganti V S, Tejero M E, Delgado-Enciso I, Pérez-Ibave D C, Schlabritz-Loutsevitch N E, Mohamed-Noriega J, Martinez-Fierro M L, Reséndez-Pérez D, Cole S A, Cavazos-Adame H, Comuzzie A G, Mohamed-Hamsho J, Barrera-Saldaña H A
机构信息
Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.
出版信息
J Med Primatol. 2013 Jun;42(3):105-11. doi: 10.1111/jmp.12037. Epub 2013 Feb 9.
BACKGROUND
Olfactomedin-like is a family of polyfunctional polymeric glycoproteins. This family has at least four members. One member of this family is OLFML3, which is preferentially expressed in placenta but is also detected in other adult tissues including the liver and heart. However, its orthologous rat gene is expressed in the iris, sclera, trabecular meshwork, retina, and optic nerve.
METHODS
OLFML3 messenger amplification was performed by RT-PCR from human and baboon ocular tissues. The products were cloned and sequenced.
RESULTS
We report OLFML3 expression in human and baboon eye. The full coding DNA sequence has 1221 bp, from which an open reading frame of 406 amino acid was obtained. The baboon OLFML3 gene nucleotidic sequence has 98% and amino acidic 99% similarity with humans.
CONCLUSIONS
OLFML3 gene expression in human and baboon ocular tissues and its high similarity make the baboon a powerful model to deduce the physiological and/or metabolic function of this protein in the eye.
背景
嗅觉介质样蛋白是一类多功能聚合糖蛋白家族。该家族至少有四个成员。其中一个成员是OLFML3，它在胎盘中优先表达，但在包括肝脏和心脏在内的其他成人组织中也可检测到。然而，其直系同源大鼠基因在虹膜、巩膜、小梁网、视网膜和视神经中表达。
方法
通过RT-PCR从人和狒狒的眼部组织中进行OLFML3信使扩增。对产物进行克隆和测序。
结果
我们报道了OLFML3在人和狒狒眼中的表达。完整的编码DNA序列有1221bp，从中获得了一个406个氨基酸的开放阅读框。狒狒的OLFML3基因核苷酸序列与人类的相似性为98%，氨基酸相似性为99%。
结论
OLFML3基因在人和狒狒眼部组织中的表达及其高度相似性使得狒狒成为推断该蛋白在眼中的生理和/或代谢功能的有力模型。
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