Laboratorio de Producción Animal, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco, Chile; Programa de Doctorado en Ciencias mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Temuco, Chile.
Andrologia. 2014 Mar;46(2):194-7. doi: 10.1111/and.12070. Epub 2013 Feb 7.
Seminal plasma contains various biochemical components associated with sperm function. However, there is limited information regarding the fatty acid composition of seminal plasma and their effect on sperm. The aim of this study was to identify the fatty acid content in canine seminal plasma using gas chromatography. Twelve ejaculates were studied, the seminal plasma was obtained by centrifugation and then the lipids were extracted, methylated and analysed by chromatography. The total lipids in the seminal plasma were 2.5 ± 0.3%, corresponding to 85% saturated fatty acids (SFA) and 15% unsaturated fatty acids (UFA). The greatest proportions of SFA were palmitic acid (30.4%), stearic acid (23.4%) and myristic acid (5.3%) and of UFA oleic acid (9.0%). Therefore, the protocols and techniques used enabled the identification of 18 different fatty acids in canine seminal plasma, which constitutes a good method to evaluate and quantify the fatty acid profile in this species.
精浆含有与精子功能相关的各种生化成分。然而，关于精浆的脂肪酸组成及其对精子的影响的信息有限。本研究旨在使用气相色谱法鉴定犬精浆中的脂肪酸含量。研究了 12 个射精，通过离心获得精浆，然后提取脂质，甲酯化并通过色谱分析。精浆中的总脂质为 2.5±0.3%，对应 85%饱和脂肪酸 (SFA) 和 15%不饱和脂肪酸 (UFA)。SFA 中比例最大的是棕榈酸 (30.4%)、硬脂酸 (23.4%) 和肉豆蔻酸 (5.3%)，UFA 中比例最大的是油酸 (9.0%)。因此，使用的方案和技术能够鉴定犬精浆中的 18 种不同的脂肪酸，这是评估和定量该物种脂肪酸谱的一种很好的方法。
