Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Jinan 250012, Shandong, China; Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China; CNPC Jichai Power Equipment Company Hospital, Jinan, Shandong, China.
Clin Nutr. 2013 Oct;32(5):849-54. doi: 10.1016/j.clnu.2013.01.008. Epub 2013 Jan 23.
BACKGROUND & AIMS: Chemokine CXC ligand 16 (CXCL16) has chemokine, adhesion molecule and scavenger receptor functions involving the immune function. Atherosclerosis is an inflammatory disease. We aimed to study the association of chemokine CXCL16/CXCR6 and carotid atherosclerosis in patients with metabolic syndrome.
Carotid ultrasonography was determined in 30 patients with metabolic syndrome and 30 controls. The mRNA levels of CXCL6/CXCR6 were detected by real-time RT-PCR. The activation of T cells and expression of CXCR6 in T lymphocyte cells and natural killer T (NKT) cells was detected by flow cytometry. The serum level of sol-CXCL6 was determined by ELISA.
Compared with controls, patients with metabolic syndrome showed significantly increased waist circumference and levels of total cholesterol, triglycerides and low-density lipoprotein cholesterol (all P < 0.001), with increased abnormalities of the structure and function of the carotid artery (P < 0.05). In metabolic syndrome, the levels of sol-CXCL16 and CXCL16mRNA were increased and associated with max IMT and plaque index. Patients with metabolic syndrome showed increased number of CXCR6+ T cells and CXCR6+ NKT cells, which was associated with max IMT and plaque index.
CXCL16 and CXCR6 may be associated the formation of carotid atherosclerotic plaque in metabolic syndrome, and T cells may be the important effector cells in the pathogenesis of the atherosclerosis.
趋化因子 CXC 配体 16（CXCL16）具有趋化因子、黏附分子和清道夫受体功能，涉及免疫功能。动脉粥样硬化是一种炎症性疾病。我们旨在研究代谢综合征患者趋化因子 CXCL16/CXCR6 与颈动脉粥样硬化的相关性。
对 30 例代谢综合征患者和 30 例对照者进行颈动脉超声检查。采用实时 RT-PCR 检测 CXCL6/CXCR6 的 mRNA 水平。通过流式细胞术检测 T 淋巴细胞和自然杀伤 T（NKT）细胞中 T 细胞的激活和 CXCR6 的表达。通过 ELISA 法测定血清可溶性 CXCL16（sol-CXCL16）水平。
与对照组相比，代谢综合征患者的腰围和总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平明显升高（均 P<0.001），颈动脉结构和功能异常增多（P<0.05）。在代谢综合征中，sol-CXCL16 和 CXCL16mRNA 水平升高，与 max IMT 和斑块指数相关。代谢综合征患者 CXCR6+T 细胞和 CXCR6+NKT 细胞数量增加，与 max IMT 和斑块指数相关。
CXCL16 和 CXCR6 可能与代谢综合征颈动脉粥样硬化斑块的形成有关，T 细胞可能是动脉粥样硬化发病机制中的重要效应细胞。
