Life Science Research Center, Beihua University, Jilin 132013, China.
Carbohydr Polym. 2013 Feb 15;92(2):1071-7. doi: 10.1016/j.carbpol.2012.10.059. Epub 2012 Oct 30.
Although neutrophils play important roles in host defense, sometimes they contribute to inflammation-related tissue injuries. Thus, restriction of their recruitment to the inflammatory sites is a promising strategy in the amelioration of inflammatory disease. The previous studies reported the anti-inflammatory effects of Bupleurum chinense, but the active ingredients and possible mechanism are still unclear. Here, we isolated water-soluble polysaccharides (BCPs) from B. chinense, to evaluate its anti-inflammatory effects and possible mechanism. The present results showed that BCPs significantly impaired the in vivo neutrophil infiltration, as well as the migration capacity of dHL-60 cells in vitro. In addition, BCPs inhibits chemoattractant fMLP-induced activation and clustering of β2 integrin. BCPs impacted fMLP-induced actin polymerization and the activation of cytoskeleton regulatory molecules, Vav1 and Rac1. Together, BCPs significantly impacted recruitment and migration of neutrophils by blocking chemoattractant receptor-mediated functions, and it possesses a potential as novel anti-inflammatory drug.
虽然中性粒细胞在宿主防御中发挥着重要作用，但有时它们也会导致炎症相关的组织损伤。因此，限制它们向炎症部位募集是改善炎症性疾病的一种有前途的策略。先前的研究报道了柴胡的抗炎作用，但活性成分和可能的机制仍不清楚。在这里，我们从柴胡中分离出水溶性多糖（BCPs），以评估其抗炎作用和可能的机制。目前的结果表明，BCPs 可显著抑制体内中性粒细胞浸润以及体外 dHL-60 细胞的迁移能力。此外，BCPs 抑制趋化因子 fMLP 诱导的β2 整合素的激活和聚集。BCPs 影响 fMLP 诱导的肌动蛋白聚合和细胞骨架调节分子 Vav1 和 Rac1 的激活。综上所述，BCPs 通过阻断趋化因子受体介导的功能显著影响中性粒细胞的募集和迁移，具有作为新型抗炎药物的潜力。
