Department of Chemical Engineering, Ichinoseki National College of Technology, Ichinoseki, Iwate, 021-8511, Japan.
Carbohydr Polym. 2013 Feb 15;92(2):1573-8. doi: 10.1016/j.carbpol.2012.10.068. Epub 2012 Nov 12.
This study examined the effects of a combined pretreatment with supercritical water and mechanochemical grinding with a ball mill on the physicochemical properties of chitin and its enzymatic degradation. Following pretreatment with a combination of supercritical water and grinding, chitin had a lower mean molecular weight, a lower crystallinity index, a lower crystallite size, greater d-spacing, weaker hydrogen bonds, and the amide group was more exposed compared with untreated chitin. These properties increased the hydrophilicity of the chitin and enhanced its enzymatic degradation. The N,N'-diacetylchitobiose (GlcNAc)(2) yield after enzymatic degradation of chitin following pretreatment with supercritical water (400 °C, 1 min) and grinding (800 rpm, 10 min) was 93%, compared with 5% without any treatment, 37% with supercritical water pretreatment alone (400 °C, 1 min), and 60% with grinding alone (800 rpm, 30 min).
本研究考察了超临界水与球磨的机械化学联合预处理对壳聚糖理化性质及其酶解的影响。经过超临界水与研磨的联合预处理后，壳聚糖的重均分子量降低，结晶度指数降低，结晶尺寸减小，层间距增大，氢键减弱，酰胺基团暴露更多，与未经处理的壳聚糖相比。这些特性提高了壳聚糖的亲水性，并增强了其酶解。经过超临界水（400°C，1 分钟）和研磨（800rpm，10 分钟）预处理后，壳聚糖经酶解生成的 N,N'-二乙酰壳二糖（GlcNAc）(2)的产率为 93%，而未经任何处理的产率为 5%，单独进行超临界水预处理（400°C，1 分钟）的产率为 37%，单独进行研磨（800rpm，30 分钟）的产率为 60%。
