Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan.
Carbohydr Polym. 2013 Feb 15;92(2):2141-8. doi: 10.1016/j.carbpol.2012.11.097. Epub 2012 Dec 10.
The chitosanase and chitinase activity were revealed in the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The chitosan with 60% degree of deacetylation (DD) was depolymerized by TKU027 crude enzyme. The low DP oligomers stimulated the growth of Lactobacillus paracasei BCRC12193 and Lactobacillus kefir BCRC14011 in a MRS broth supplemented with low DP oligomers for 12 h. Conversely, the high DP oligomers (0.1%) had potent inhibitory effects against L. paracasei BCRC12193 and L. kefir BCRC14011 for 48 h. Besides, the study also investigated the effects of B. cereus TKU027 on degradation of SHP and the survival conditions of bacteria in mangrove river sediment of Tamsui River. The 5 weeks-incubation sample of SHP and B. cereus TKU027-amended mangrove river sediment showed the highest amounts of biomass, reducing sugar and total sugar, and some variance of bacterial community compositions existed in the soils.
以虾头粉（SHP）为唯一碳/氮源的蜡样芽胞杆菌 TKU027 的培养上清液中显示出壳聚糖酶和几丁质酶活性。脱乙酰度为 60%的壳聚糖被 TKU027 粗酶解聚。低 DP 寡糖在补充低 DP 寡糖的 MRS 肉汤中刺激副干酪乳杆菌 BCRC12193 和发酵乳杆菌 BCRC14011 的生长 12 h。相反，高 DP 寡糖（0.1%）对副干酪乳杆菌 BCRC12193 和发酵乳杆菌 BCRC14011 具有强烈的抑制作用，持续 48 h。此外，本研究还研究了蜡样芽胞杆菌 TKU027 对 SHP 降解和淡水河红树林沉积物中细菌生存条件的影响。5 周孵育的 SHP 样品和添加蜡样芽胞杆菌 TKU027 的红树林河沉积物显示出最高的生物量、还原糖和总糖含量，土壤中存在一些细菌群落组成的差异。
