Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung City, Taiwan.
Life Sci. 2013 Apr 9;92(12):719-26. doi: 10.1016/j.lfs.2013.01.027. Epub 2013 Feb 8.
The aim of this study is to investigate the protective effects of cystamine on lupus-associated cardiac hypertrophy.
Balb/c and lupus-prone NZB/W-F1 mice were individually randomized into sham group (saline, n=16) and cystamine group (n=16). Mice received saline or cystamine (100 mmol in 100 μL saline) by daily intraperitoneal injection for 2 consecutive weeks. Morphological, histological, and biochemical alterations were investigated.
Cystamine decreased both left ventricular (LV) mass and LV mass/tissue-to-blood ratio (TBR) in NZB/W-F1 mice (p<0.05), whereas slight effects were observed in Balb/c mice. Moreover, cystamine reduced levels of atrial natriuretic peptide (ANP), C-reactive protein (CRP), heart type-fatty acid binding protein (h-FABP), creatine kinase-MB (CK-MB) and IL-6 in LV tissues of NZB/W-F1 mice (p<0.05). Additionally, in LV tissues of NZB/W-F1 mice, suppression of hypertrophic signaling mediated by IL-6 in response to administration of cystamine was revealed, including phosphorylation of MEK5, ERK5, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38) (p<0.05).
Cystamine alleviated LV hypertrophy in NZB/W-F1 mice as a result of decrease in hypertrophic mediators and suppression of IL-6 mediated hypertrophic signaling.
本研究旨在探讨胱胺对狼疮相关性心肌肥厚的保护作用。
将 Balb/c 小鼠和狼疮易感 NZB/W-F1 小鼠分别随机分为假手术组（生理盐水，n=16）和胱胺组（n=16）。小鼠每日腹腔注射生理盐水或胱胺（100 μL 生理盐水含 100 mmol），连续 2 周。观察形态学、组织学和生化改变。
胱胺降低了 NZB/W-F1 小鼠的左心室（LV）质量和 LV 质量/组织与血液比（TBR）（p<0.05），而对 Balb/c 小鼠的影响较小。此外，胱胺降低了 NZB/W-F1 小鼠 LV 组织中心房利钠肽（ANP）、C 反应蛋白（CRP）、心脏型脂肪酸结合蛋白（h-FABP）、肌酸激酶-MB（CK-MB）和白细胞介素-6（IL-6）的水平（p<0.05）。此外，在 NZB/W-F1 小鼠的 LV 组织中，发现胱胺抑制了 IL-6 介导的肥厚信号，包括 MEK5、ERK5、c-Jun N 端激酶（JNK）和 p38 丝裂原活化蛋白激酶（p38）的磷酸化（p<0.05）。
胱胺通过降低肥大介质和抑制 IL-6 介导的肥大信号，减轻了 NZB/W-F1 小鼠的 LV 肥厚。
