Direct DNA sequencing of PCR amplified genomic DNA by the Maxam-Gilbert method.
作者信息
Tahara T, Kraus J P, Rosenberg L E
机构信息
Yale University School of Medicine, Department of Human Genetics, New Haven, CT 06510.
出版信息
Biotechniques. 1990 Apr;8(4):366-8.
PMID:2340169
Abstract
We present a method to directly sequence PCR amplification products utilizing the chemical method of Maxam and Gilbert. This procedure yields clearly readable DNA sequences of 100-400 base pairs in length derived from human genomic DNA in four days' time.
摘要
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