Cornel Anthony, Lee Yoosook, Fryxell Rebecca Trout, Siefert Stephanie, Nieman Catelyn, Lanzaro Gregory
Mosquito Control Research Laboratory, Department of Entomology, University of California at Davis, 9240 S. Riverbend Avenue, Parlier, 93648 California, USA.
J Am Mosq Control Assoc. 2012 Dec;28(4 Suppl):113-21. doi: 10.2987/8756-971X-28.4s.113.
Culex pipiens sensu lato populations represent significant nuisance pests and vectors of West Nile virus in California. Despite multiple years of investigation, identifying, controlling and understanding the behaviors and associated "biologies" of the complex members still remain a challenge. Population structure cluster analysis using microsatellite markers revealed extensive population structuring, particularly in the central parts of the State, over and above what can be explained by the presence of Cx. pipiens, Cx. quinquefasciatus and their hybrids. Ace 2 gene sequencing provided evidence for the presence of Cx. p. molestus in multiple locations both above and below ground in California. Lack of congruence of male genitalia morphology (dorsal and ventral arms/dorsal arm of phalosome) and polymerase chain reaction diagnostic assay identifications coupled with considerable heterozygosity of pyrethroid resistance in time and space all suggest complex population structuring not adequately explained using current concepts.
致倦库蚊复合组种群是加利福尼亚州重要的滋扰性害虫和西尼罗河病毒的传播媒介。尽管经过多年调查，但识别、控制并了解该复合组成员的行为及相关“生物学特性”仍是一项挑战。利用微卫星标记进行的种群结构聚类分析显示出广泛的种群结构，特别是在该州中部地区，其程度超过了致倦库蚊、致乏库蚊及其杂交种的存在所能解释的范围。Ace 2基因测序为加利福尼亚州多个地上和地下位置存在骚扰库蚊提供了证据。雄性生殖器形态（阳茎体的背臂和腹臂/背臂）与聚合酶链反应诊断检测鉴定结果不一致，再加上拟除虫菊酯抗性在时间和空间上存在相当大的杂合性，所有这些都表明目前的概念无法充分解释复杂的种群结构。
